Induce pluripotent stem cells using a single microRNA cluster
Use lentivirus, PiggyBac or minicircle DNA
- miR-302bcad/367 cluster
- Lentivector features GFP+Puro
- Pre-packaged lentivirus ready to transduce
- Minicircle vector co-expresses GFP
- Pre-made minicircle DNA ready to transfect
Overexpress the Stem-cell specific microRNA-302bcad/367 cluster with high efficiency
Induce either human or mouse somatic cells into the pluripotent state using a single microRNA cluster expression vector. The Human or Mouse microRNA302bcad/367 expression cassettes are available in Lentivector and PiggyBac formats as well as non-integrating Minicircle DNA vector formats.
The microRNA-based reprogramming method has been published to be more efficient that the standard technique based on Oct4/Sox2/Klf4/Myc reprogramming transcription factors. The microRNA-derived iPSCs display similar morphologies, pluripotent marker expression, and teratoma formation, and the mouse miR-iPSCs contribute to successful chimera germline transmission.
See the landmark publications here for experimental details:
Anokye-Danso F, Trivedi CM, Juhr D, Gupta M, Cui Z, Tian Y, Zhang Y, Yang W, Gruber PJ, Epstein JA, Morrisey EE. Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency. Cell Stem Cell. 2011 Apr 8;8(4):376-88.
Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, Pei D. MicroRNA cluster 302-367 enhances somatic cell reprogramming by accelerating a mesenchymal-to-epithelial transition. J Biol Chem. 2011 May 13;286(19):17359-64.