MicroRNA Reprogramming
Induce pluripotent stem cells using a single microRNA cluster
Use lentivirus, PiggyBac or minicircle DNA
- miR-302bcad/367 cluster
- Lentivector features GFP+Puro
- Pre-packaged lentivirus ready to transduce
- Minicircle vector co-expresses GFP
- Pre-made minicircle DNA ready to transfect
Online Resources
Technical References
Anokye-Danso F, Trivedi CM, Juhr D, Gupta M, Cui Z, Tian Y, Zhang Y, Yang W, Gruber PJ, Epstein JA, Morrisey EE. Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency. Cell Stem Cell. 2011 Apr 8;8(4):376-88.
Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, Pei D. MicroRNA cluster 302-367 enhances somatic cell reprogramming by accelerating a mesenchymal-to-epithelial transition. J Biol Chem. 2011 May 13;286(19):17359-64.