PSGro® hESC/iPSC Growth Medium

• Serum-free
• Xeno-free
• Feeder-free
• Worry-free

Catalog# SC500M-1

Basic techniques to culture hESCs and generate hiPSCs have been well developed, although limitations remain in many aspects. Currently, many hESC/hiPSC lines have been cultured using mouse or human fibroblast feeders and serum or conditioned medium. These methods are labor intensive, and can prevent clinical applications due to the use of animal derived components.
System Biosciences and StemRD together have developed PSGro hESC/iPSC Growth Medium, which is a chemically-defined medium for feeder-independent maintenance of hESCs and hiPSCs. It is a complete, serum-free, defined formulation. PSGro hESC/iPSC Growth Medium has been tested and proven to support the growth and maintenance of pluripotency of both hESC and hiPSC lines when used with BD Matrigel™ hESC qualified Matrix (BD Catalog #354277) as a substrate.
PSGro® hESC/iPSC Growth Medium is chemically defined and xeno-free, thus eliminating concerns of lot-to-lot variations and potential animal-borne pathogens.

Maintain Morphology and Pluripotency Marker Expression

Morphology of hESCs (A,B) & hiPSCs (C,D) Cultured in PSGro medium (Left Panel). Human iPSCs grown in PSGro medium express pluripotency markers. Immunofluorescence analysis of hiPSCs shows expression of (E) Oct4, (F) TRA-1-81, (G) Nanog and (H) SSEA-3 (Right Panel).

Retain Normal Karyotype and Differentiation Potential

Chromosomal analysis of hiPSCs cultured in PSGro medium for 28 passages shows a normal male karyotype 46 XY (Left Panel). Human iPSCs were cultured in PSGro medium and then injected subcutaneously into SCID mice. Resulting teratomas contained cell types from all 3 germ layers (Right Panel).

Media supplements and Growth factors also available