Directly express protein factors using pre-made mRNAs

The mRNAExpress™ transcripts are mRNAs synthesized in vitro. The mRNAs have enhanced stability by incorporating modified nucleobases and offer increased translatability with 5' cap analogs and 3' polyA tails. The mRNAs can be delivered through transfection into a broad range of cell types, including fibroblasts and lymphocytes. This system enables a titratable, reproducible and non-integrating method for directly expressing key cellular factors involved in generating iPS cells as well as direct differentiation factors. Protein expression from the mRNAs can be seen within 5 hours after transfection with maximum protein expression observed between 24 to 48 hours. Pre-made mRNA transcripts - ready for transfection.

How the mRNAExpress transcripts are made