iPSC Reprogramming Factors
For induction of pluripotent stem cells
Study pluripotency
- Oct4
- Nanog and Sox2
- KLF4 or Lin28
- c-Myc
Retrovirus Features and Benefits
- Standard Retroviral-based protein expression system
- OSKM Pluripotency factors expressed
Mouse iPS cell colony formation.
The mouse iPS cells were reprogrammed from fibroblasts
by using Retroviruses for Oct4, Sox2, cMyc and Klf4. The cells were validated for pluripotent stem cell markers Nanog and SSEA-1 staining. The video shows Mouse iPS cells that were seeded on the glass bottom dish with MEF feeder cells and visualized through a 20X objective lens. The images were taken every 15 minutes for 72 hours at 800x600 resolution with an 1/180 second exposure time. Video created using the Nikon BioStation IM Live Cell Recorder