Stem Cell Research iPSC Reprogramming Factors

Overview
Technical Details
Literature
Ordering

Induced Pluripotency Stem Cell (iPSC) Factor Constructs

Six iPSC Factors for Stem Cell pluripotency reprogramming—Human clones ready-made and expression validated.

iPSC Factor Expression Vector

Six iPSC Factors for Stem Cell Reprogramming

Oct4 ● SOX2

Nanog ● KLF4

Lin-28 ● c-Myc

"The Magic Brew"—Rossant, 2007

Features and Benefits

Lentiviral-based protein expression system, HIV backbone for high viral titers
Pluripotency factors expressed from constitutive EF1α promoter
Reliable delivery to dividing or non-dividing cells
Monitor positive expressing cells with co-expressed RFP
All proteins are N-terminal HA-tagged
Convenient sorting of transfected or transduced cells
Create stable cell lines
Monitor positive cells with RFP
All 6 iPSC factors expression-validated

Transfection Data

Protein expression Western Blot Data

The piPSC constructs can be used in transient transfection experiments (shown above) or can be used to package lentiviral particles. The particles can then be used to transduce any cell type to create stable cell lines permanently expressing the iPSC protein of interest. Examples of VSV-g pseudotyped lentiviral transductions are shown below.

Transduce Dividing and Non-dividing cells
piPSC-Nanog activates Nanog reporter transcription.

Lentiviral transduction data

Nanog reporter cell line transactivation data

Kit Components

Each kit contains 10 ug of endotoxin-free plasmid DNA in TE buffer or 1 x 10^6 ifu/ml pre-packaged lentivirus.
Each kit comes with a product certificate of analysis.

Pluripotency Factor Technical References

Rossant, J. 2007. Stem cells: The magic brew. Nature 448, 260–262.

Takahashi, K. and Yamanaka, S. 2006. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126: 663–676.

Takahashi K. et al. 2007. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 131: 861–72.

Park, IH et al. 2008. Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 451:141–6.

Baker, Monya 2007. Adult cells reprogrammed to pluripotency, without tumors. Nature Reports Stem Cells. 2007–12–11.

Nakagawa, M et al. 2008. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nature Biotechnology. 26: 101–106.

Okita, K, et al. 2007. Generation of germline-competent induced pluripotent stem cells. Nature. 448:313–7

Yu, J. et al. 2007. Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science 318: 1917–1920.

Pre-packaged lentivirus

Quantity	Product	Size	Catalog #	Price
	pPS-human Nanog-T2A-RFP Construct [pre-packaged]	>2 x 10^6 IFUs	SR10060VA-1	$519.75
	pPS-human Oct4-T2A-RFP Construct [pre-packaged]	>2 x 10^6 IFUs	SR10061VA-1	$519.75
	pPS-human Sox2-T2A-RFP Construct [pre-packaged]	>2 x 10^6 IFUs	SR10062VA-1	$519.75
	pPS-human c-Myc-T2A-RFP Construct [pre-packaged]	>2 x 10^6 IFUs	SR10063VA-1	$519.75
	pPS-human Lin28-T2A-RFP Construct [pre-packaged]	>2 x 10^6 IFUs	SR10064VA-1	$519.75
	pPS-human KLF4-T2A-RFP Construct [pre-packaged]	>2 x 10^6 IFUs	SR10065VA-1	$519.75
	iPSC Kit (Nanog, Oct4, Sox2, cMyc, Lin28, KLF4) virus	>2 x 10^6 IFUs of each	SR10066VA-1	$3,045.00
	iPSC Y Pre-packaged Set (c-Myc, Oct4, Sox2, KLF4)	>2 x 10^6 IFUs of each	SR10076VA-1	$1,825.00
	iPSC T Pre-packaged Set (Lin28, Nanog, Oct4, Sox2)	>2 x 10^6 IFUs of each	SR10077VA-1	$1,825.00

Plasmid lentivectors

Quantity	Product	Size	Catalog #	Price
	pPS-human Nanog-T2A-RFP Construct [plasmid]	10 ug	SR10060PA-1	$1,359.75
	pPS-human Oct4-T2A-RFP Construct [plasmid]	10 ug	SR10061PA-1	$1,359.75
	pPS-human Sox2-T2A-RFP Construct [plasmid]	10 ug	SR10062PA-1	$1,359.75
	pPS-human cMyc-T2A-RFP Construct [plasmid]	10 ug	SR10063PA-1	$1,359.75
	pPS-human Lin28-T2A-RFP Construct [plasmid]	10 ug	SR10064PA-1	$1,359.75
	pPS-human KLF4-T2A-RFP Construct [plasmid]	10 ug	SR10065PA-1	$1,359.75
	iPSC Kit (Nanog, Oct4, Sox2, cMyc, Lin28, KLF4) plasmids	10 ug of each plasmid	SR10066PA-1	$8,085.00
	pPS-T2A-RFP Control for iPSC Factors [plasmid]	10 ug	SR10071PA-1	$450.00
	iPSC Y Plasmid Set (c-Myc, Oct4, Sox2, KLF4)	10 ug of each plasmid	SR10076PA-1	$4,850.00
	iPSC T Plasmid Set (Lin28, Nanog, Oct4, Sox2)	10 ug of each plasmid	SR10077PA-1	$4,850.00

• VA-1 product version refers to virus (>2 x 10^6 IFUs)
• PA-1 product version refers to plasmid (10 ug)
• iPSC Kit (entire set) comes with 6 individual plasmids or 6 individual pre-packaged lentiviral preparations.

iPSC plasmid sequence information can be found in the Stem Cell Resources Section.
Stem Cell Resources Section

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iPSC Reprogramming Factors

For induction of pluripotent stem cells

Study pluripotency