iPS Cell Lines

Matched-sets of Human and Mouse iPS Cell Lines

Jump start your iPS cell research with pre-made, high quality iPS cell lines
• iPS cell lines made using Oct4, Sox2, c-Myc, and KLF4
• Study the induced pluripotency process
• Measure mRNA and microRNA signatures
• Identify novel epigenetic markers to study pattern formation
• Develop directed differentiation protocols
• Have SBI create custom iPS cell lines, please inquire: info@systembio.com

• Human certified iPS Cell Line catalog# SC101A-1
• Mouse certified iPS Cell Line catalog# SC201A-1

All iPS Cell lines are certified to be pluripotent through iPS Cell Marker staining

Stem cell markers for SSEA4/1 and Nanog were determined by immunocytochemistry using primary antibodies for SSEA1 (Millipore), SSEA4 (Abcam) and Nanog (Abcam) followed by fluorescent-labeled secondary Alexa594 antibodies (Invitrogen). Detection of Alkaline Phosphatase activity was performed using the AP Detection kit (Millipore). Teratoma assays were performed by subcutaneous injection of 1x10^6 iPS cells into the dorsal flank of SCID mice. Teratomas were visualized after 4 weeks with hematoxylin and eosin staining.

How the iPS Cell Lines are made

Human foreskin fibroblasts (HFFs) or Mouse embryonic fibroblasts (MEFs) are transduced with high-titer iPSC "clean" virus to induce pluripotency. The result is high quality iPS cells with no fluorescent markers or drug selection required. The entire process takes up to 6 weeks and these iPS cell lines are pre-made and validated - ready for your research.

iPS Cell Line Differentiation Studies

Mouse iPS Cells are capable of producing multiple neural lineages

• Develop directed differentiation protocols
• Make new transgenic lines
• Confirm lineage specification using SBI's Differentiation Lenti-reporters

Quality Source and Feeder Cells for Reliable Reprogramming

Human Epidermal Keratinocytes, neonatal cells only or complete kit
• Highly pure, passage 1 neonatal epidermal keratinocytes optimized for iPSC generation
• Isolated from individual foreskin, single genetic background
• Human epidermal keratinocytes have been demonstrated to reprogram at least 100-fold more efficiently and at twice the speed of fibroblasts
• Keratinocytes are the only cell type of ectodermal origin - best source cells for Neural studies

Product formats

• Neonatal Epidermal Keratinocyte Complete Kit (Cat#PC503hEKTN-K) for iPSC generation includes keratinocytes, culture medium and neutralization solution to jump-start your reprogramming

• Cells only option (Cat#PC503hEKTN-C)

Human Epidermal Keratinocytes (Primary cells, Passage 1)
Note the "cobblestone-like" morphology

Human foreskin fibroblasts, neonatal (Cat#PC501A-HFF)
• Highly pure neonatal foreskin fibroblasts optimized for iPSC generation
• Isolated from individual foreskin, single genetic background
• Measure mRNA and microRNA signatures
• Two times more cells/vial compared with competitors’ product

Highly pure neonatal foreskin fibroblasts for Feeder Cells (Cat#PC502B-HFF)
• Highly pure neonatal foreskin fibroblasts
• Pooled neonatal foreskin fibroblasts optimized as feeder cells

Human Foreskin Fibroblasts (Primary cells, Passage 1)

Online Resources

Technical References

Rossant, J. 2007. Stem cells: The magic brew. Nature 448, 260–262.

Takahashi, K. and Yamanaka, S. 2006. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126: 663–676.

Takahashi K. et al. 2007. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 131: 861–72.

Park, IH et al. 2008. Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 451:141–6.

Baker, Monya 2007. Adult cells reprogrammed to pluripotency, without tumors. Nature Reports Stem Cells. 2007–12–11. 

Nakagawa, M et al. 2008. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nature Biotechnology. 26: 101–106.

Okita, K, et al. 2007. Generation of germline-competent induced pluripotent stem cells. Nature. 448:313–7

Yu, J. et al. 2007. Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science 318: 1917–1920.

Human and Mouse iPS Cell Line Sets Quantity Product Size Catalog # Price Human iPS Cell Line Set [HFF and iPS cells] 2x10^5 cells each SC101A-1 $1,045.00 Mouse iPS Cell Line Set [MEF and iPS cells] 2x10^5 cells each SC201A-1 $845.00 The pre-made iPS Cell lines are available as matched sets of the source fibroblast cells along with their induced pluripotent cells. To learn more about custom iPS Cell Line generation, please contact SBI. Send SBI your cells of interest, we reprogram them into the pluripotent state. Email SBI today to get started: info@systembio.com Related iPSC Products: Source Cells, Feeder Cells and Stem Cell Media Quantity Product Size Catalog # Price Human Foreskin Fibroblasts for iPSC Construction (Passage P1, from individual genetic source) 1x10^6 cells PC501A-HFF $395.00 Human Foreskin Fibroblasts as Feeder Cells (Passage P1, pooled samples) 1x10^6 cells PC502B-HFF $279.00 Human Epidermal Keratinocytes (Cryopreserved) for iPSC 1x10^6 cells PC503hEKTN-C $395.00 Human Epidermal Keratinocytes for iPSC Construction (Kit) 1x10^6 cells PC503hEKTN-K $795.00 StemPure™ ESC/iPSC Growth Medium (500ml) 500 ml SC050M-1 $159.00 Highly pure Neonatal foreskin fibroblasts optimized for iPSC genereation (cat# PC501A-HFF) Pooled Neonatal foreskin fibroblasts optimized for use as feeder cells (cat# PC502B-HFF) Highly reprogrammable Human epidermal keratinocytes kit (cat# PC503hEKTN-K) or cells only (cat# PC503hEKTN-C) Neonatal foreskin fibroblasts optimized for use as feeder cells (cat# PC502B-HFF) Grow your embryonic and iPS cells reliably in SBI's StemPure™ media (cat# SC050M-1)

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