Monitor Stem Cell Differentiation in Real-time

Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4;3(6):670-80.

Live imaging of neuronal differentiation
The periodic "flashes" seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The entire differentiation process from mouse neural stem cell progenitors to form the network of mature neurons, oligodendrocytes and astrocytes took approximately 7 days. SBI's mGFAP lenti-reporter for the Glial Fibrillary Acidic Protein promoter(cat.#SR10016VA-1) reporter was used to track the development of astrocytes (bright gray in the video). The final photo taken after the network formation is shown below (color added). Only the astrocytes are very bright green among the network of neurons demonstrating the specificity of SBI's mGFAP lenti-reporter.

Final live-cell photo of mGFAP reporter for Astrocytes

Simultaneously Track Multiple Lineages from iPS and Progenitor Cells

Additional Differentiation Reporter Data