• Induce Pluripotency with iPSC factors
• Study Pluripotency with pre-made iPS Cell lines
• Confirm Pluripotency with Oct4 and Nanog
• Track Differentiation across 5 lineages
These tools can be used to induce and monitor the pluripotent state and track stem cells during differentiation.
Mouse iPS cell colony formation.
The mouse iPS cells were reprogrammed from fibroblasts
by using Oct4, Sox2, cMyc and Klf4. The cells were
validated for pluripotent stem cell markers Nanog
and SSEA-1 staining. The video shows Mouse iPS
cells that were seeded on the glass bottom dish
with MEF feeder cells and visualized through
a 20X objective lens. The images were taken every 15 minutes
for 72 hours at 800x600 resolution with an 1/180 second exposure time.
Video created using the Nikon BioStation IM Live Cell Recorder
