• Induce Pluripotency with iPSC factors
• Study Pluripotency with pre-made iPS Cell lines
• Confirm Pluripotency with Oct4 and Nanog
• Track Differentiation across 5 lineages
These tools can be used to induce and monitor the pluripotent state and track stem cells during differentiation.
Mouse iPS cell colony formation.
The mouse iPS cells were reprogrammed from fibroblasts
by using Oct4, Sox2, cMyc and Klf4. The cells were
validated for pluripotent stem cell markers Nanog
and SSEA-1 staining. The video shows these mouse iPS
cells seeded on the glass bottom dish with MEF feeder
cells and visuallized through a 20X objective lens.
The images were taken every 15 min for 72 hours at
800x600 resolution, 1/180 s of exposure time.
Video created using the Nikon BioStation IM Live Cell Recorder
