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Stem Cell Research

Tools to study pluripotency and differentiation

iPSC Technologies and Reporting Systems

  • Induce Pluripotency with iPSC factors
  • Study Pluripotency with pre-made iPS Cell lines
  • Confirm Pluripotency with Oct4 and Nanog
  • Track Differentiation across 5 lineages

Induce Pluripotency

Create novel iPS Cell Lines using nonviral or retroviral systems.

iPS Cell lines

Study the pluripotent state using matched source and iPS cell lines.

Confirm Pluripotency

SBI offers transcriptional reporters for Nanog and Oct4.

Transdifferentiation Factors

Directly Reprogram cells with transcription factors and microRNAs.

Track Differentiation

Track Stem cell differentiation, choose lentivector reporters from:
• Neural
• Hematopoietic
• Myogenic
• Structural
• Signaling

These tools can be used to induce and monitor the pluripotent state and track stem cells during differentiation.

Track Neural differentiation by qPCR with the Neural Lineage qPCR Profiler Array

Growth Factors, Media Separation and Cryopreservation

High quality growth factors and media
• Wnt and Hedgehog factors
• TGF beta and Notch factors
• PSGro® hESC/iPSC media
• MesenGro® media • Gentle-Stem™ dissociation reagent
• CRYO-GOLD™ cryopreservation medium

 

Mouse iPS cell colony formation. The mouse iPS cells were reprogrammed from fibro-blasts by using Oct4, Sox2, cMyc and Klf4. The cells were validated for pluripotent stem cell markers Nanog and SSEA-1 staining. The video shows Mouse iPS cells that were seeded on the glass bottom dish with MEF feeder cells and visualized through a 20X objective lens. The images were taken every 15 minutes for 72 hours at 800x600 resolution with an 1/180 second exposure time.


Video created using the Nikon BioStation IM Live Cell Recorder