Custom Human and Mouse iPS Cell Lines
Jump start your iPS cell research with custom-made, high quality iPS cell lines
• iPS cell lines generated from your model system
• iPS cell lines made using Oct4, Sox2, c-Myc, and KLF4
• Study the induced pluripotency process
• Measure mRNA and microRNA signatures
• Identify novel epigenetic markers to study pattern formation
• Develop directed differentiation protocols
• Have SBI create custom iPS cell lines, please inquire: services@systembio.com
Service Details
- Method: Retroviral delivery of Oct4, Sox2, cMyc, and Klf4 reprogramming factors. Morphological selection of colonies [no reporter genes used].
- Customer provides: Customer provides 2 vials of each cell type. Standard service is for reprogramming fibroblast cells, such as MEF or TTF (mouse) and HFF (human). Other easy to grow cells, such as epithelial cells, should also be fine. Difficult to grow cells, such as neurons, will be evaluated on a case by case basis, and may cost more than the standard service.
- SBI provides: 5 validated iPSC colonies for each genotype (>2 x 10^5 cells / colony).
- Validation: SSEA-1/4 and Nanog immunostaining to demonstrate pluripotency.
- Timeline:
- Mouse: 2-3 months [reprogramming and expansion requires a minimum of 1 month]
- Human: 3-4 months [reprogramming and expansion requires a minimum of 2 months]
All iPS Cell lines are certified to be pluripotent through iPS Cell Marker staining
Stem cell markers for SSEA4/1 and Nanog will be verified by immunocytochemistry using primary antibodies for SSEA1 (Millipore), SSEA4 (Abcam) and Nanog (Abcam) followed by fluorescent-labeled secondary Alexa594 antibodies (Invitrogen).
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