PiggyBac Transposon Services

The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites into TTAA chromosomal sites. The powerful activity of the PiggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes. PiggyBac vector services are available through a partnership between SBI and Transposagen.

PiggyBac Technology Features

• One transfection to make transgenic cells
• Effective in Human, Mouse and Rat cells
• No cargo limit - integrate 10-100kb
• Reversible integrations with no foot-print
• All-in-one cumate switch inducible vector

Integrate multiple PB vectors simultaneously - Triple Integrations possible

The integrated PiggyBac transposons can be successfully removed, footprint-free. Simply re-transfect your stably transposed cell line with increasing amounts of the Super PiggyBac transposase expression vector to mobilize and excise the integrated transposons.

PiggyBac Technology Services

• SBI will clone your cDNA, microRNA or shRNA into your choice of PiggyBac transposon vectors in 2 weeks
• Choose from a variety of promoters, markers and Cumate switch inducible formats
• Custom cell lines can be made in 8 weeks using PiggyBac vectors
• PB transgenic rat models offered in partnership with Transposagen, Inc.

To obtain more information, order a custom service, or to inquire about a custom service not listed, contact us directly at 888-266-5066 (toll-free in North America) or 650-968-2200, or e-mail us at services@systembio.com