GeneNet™ Kinase shRNA Library
For most of the target genes, four different siRNA sequences were designed per transcript using our proprietary algorithm. The libraries are available cloned into the HIV-based pSIH1-H1-Puro shRNA Expression Vector, which confers resistance to the antibiotic puromycin. This single-promoter siRNA vector expresses cloned short-hairpin RNA (shRNA) from a single H1 RNA polymerase III promoter.
The library is available in both pre-packaged and plasmid form. The pre-packaged VSV-G pseudotyped viral stock can be used to transduce your cells of choice immediately upon receipt, or be stored at -70oC for several months. With the library in plasmid form, you can perform the pseudoviral packaging step in your own cell culture facility. Enough plasmid is provided to yield about 10^9 ifu of packaged shRNA library.
Download list of genes
Human Kinase Library (PDF) »
Product Components (for pre-packaged libraries)
| Size | Description |
| 100-200 µl | GeneNet™ shRNA Library, pre-packaged in VSV-G particles (2x10^7 ifu) |
| 50 µl | cDNA Synthesis GNH Primer (10 µM) |
| 50 µl | Fwd GNH (Forward) PCR Primer (10 µM) |
| 50 µl | Rev GNH (Reverse) PCR Primer (10 µM) |
| 200 µl | NFwd-Bio (Nested Forward Biotinylated) PCR Primer (10 µM) |
| 200 µl | NRev GNH (Nested Reverse) Universal PCR Primer (10 μM) |
| 20 µl | Positive Control DNA |
| 25 µl | pSIH1-H1·siLuc-copGFP Packaged Positive Transduction Control |
| 1 disc | CD with gene/siRNA list, and data analysis program compatible with Affymetrix data file |
Product Components (for Plasmid Libraries)
| Size | Description |
| 100-200 µl | GeneNet™ shRNA Library Plasmid DNA (200 µg) |
| 50 µl | cDNA Synthesis GNH Primer (10 µM) |
| 50 µl | Fwd GNH (Forward) PCR Primer (10 µM) |
| 50 µl | Rev GNH (Reverse) PCR Primer (10 µM) |
| 200 µl | NFwd-Bio (Nested Forward Biotinylated) PCR Primer (10 µM) |
| 200 µl | NRev GNH (Nested Reverse) Universal PCR Primer (10 μM) |
| 20 µl | Positive Control DNA |
| 25 µl | pSIH1-H1·siLuc-copGFP Packaged Positive Transduction Control |
| 1 disc | CD with gene/siRNA list, and data analysis program compatible with Affymetrix data file |
