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Application Overview

Pooled lentivirus shRNA libraries

High-throughput RNAi

  • Dissect signaling pathways
  • Discover new drug targets

Online Resources

Product Citations

Wang WH, Studach LL, Andrisani OM. Proteins ZNF198 and SUZ12 are down-regulated in hepatitis B virus (HBV) X protein-mediated hepatocyte transformation and in HBV replication. Hepatology. 2011 Apr;53(4):1137-47. (PDF) »

Malbec M, Pham QT, Plourde MB, Létourneau-Hogan A, Nepveu-Traversy ME, Berthoux L. Murine double minute 2 as a modulator of retroviral restrictions mediated by TRIM5alpha. Virology. 2010 Jul 7. [Epub ahead of print]. (PDF) »

Tsujii H, Eguchi Y, Chenchik A, Mizutani T, Yamada K, Tsujimoto Y. Screening of Cell Death Genes With a Mammalian Genome-wide RNAi Library. J Biochem. 2010 Apr 26. [Epub ahead of print].

Gumireddy K, Li A, Gimotty PA, Klein-Szanto AJ, Showe LC, Katsaros D, Coukos G, Zhang L, Huang Q. KLF17 is a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Nat Cell Biol. 2009 Oct 4. [Epub ahead of print]. (PDF) »

Xu M, Takanashi M, Oikawa K, Tanaka M, Nishi H, Isaka K, Kudo M, Kuroda M. USP15 plays an essential role for caspase-3 activation during Paclitaxel-induced apoptosis. Biochem Biophys Res Commun. 2009 Oct 16;388(2):366-71. (PDF) »

Chen Y, Cairns R, Papandreou I, Koong A, Denko NC. Oxygen consumption can regulate the growth of tumors, a new perspective on the warburg effect. PLoS One. 2009 Sep 15;4(9):e7033. (PDF) »

Yeung ML, Houzet L, Yedavalli VS, Jeang KT. A genome-wide short hairpin RNA (shRNA) screening of Jurkat T-cells for human proteins contributory to productive HIV-1 replication. J Biol Chem. 2009 May 20. [Epub ahead of print]. (PDF) »

Ossovskaya VS, Dolganov G, Basbaum AI. Loss of function genetic screens reveal MTGR1 as an intracellular repressor of beta1 integrin-dependent neurite outgrowth. J Neurosci Methods. 2009 Mar 15;177(2):322-33. (PDF) »

Huang X, Wang JY, Lu X. Systems analysis of quantitative shRNA-library screens identifies regulators of cell adhesion. BMC Syst Biol. 2008 Jun 13;2:49. (PDF) »

Hwang GW, Hayashi T, Kita K, Takahashi T, Kuge S, Naganuma A. siRNA-mediated inhibition of phosphatidylinositol glycan Class B (PIGB) confers resistance to methylmercury in HEK293 cells. J Toxicol Sci. 2007 Dec;32(5):581-3. (PDF) »

Hattori H, Zhang X, Jia Y, Subramanian KK, Jo H, Loison F, Newburger PE,Luo HR. RNAi screen identifies UBE2D3 as a mediator of all-trans retinoic acid-induced cell growth arrest in human acute promyelocytic NB4 cells. Blood, 110 (2): 640-650, 2007 (PDF) »

Useful References for RNAi Screens

How to use shRNA libraries for genetic screening

Nature. 2004 Mar 25;428(6981):427-31

Nature. 2004 Mar 25;428(6981):431-7

Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):135-40

Nature 2006 (PDF) »

shRNA Library Review