Description

The PB51x Dual Promoter Series
High levels of expression from the CMV promoter (most cell types) and PB713B-1 features the MSCV promoter (active in stem cells). The multiple cloning site (MCS) located downstream of a promoter allows for convenient cloning of your gene or microRNA of interest. Downstream of your expression cassette is an EF1alpha promoter driving the expression of either the Puro, GFP, RFP, GFP+Puro or RFP+Puro markers. The entire cassette is flanked by genomic insulator elements for stabilized expression and PiggyBac Inverted Terminal repeats for mobilization and integration.

The PB53x EF1 Series with IRES Co-expressed markers
SBI’s PiggyBac IRES expression vectors use the EF1alpha promoter to drive expression of your cDNA or microRNA cloned into the MCS along with IRES-mediated co-expression of the marker. The markers available for this PiggyBac vector series include GFP, RFP and Neo.

The PBSI505/506 shRNA expression transposon series
The PiggyBac shRNA expression vectors feature either the CMV (PBSI505A-1) or the EF1alpha promoter (PBSI506A-1) to express the GFP+Puro dual markers. The downstream H1 promoter will robustly produce shRNAs cloned into the MCS.

The Super PiggyBac Transposase expression vector
The Super PiggyBac transposase expression vector features a 5’ HS4 insulator to support robust transcription from the rPolr2A promoter. The PiggyBac transposase coding sequence has been optimized for high expression, stability and activity in mammalian cells.