Reprogram cells using the PiggyBac 4-in-1 transposon vector

The PiggyBac Mouse 4-in-1 iPSC Vector (cat# PB400A-1) features a Neo and GFP markers as well as a 2A-mediated co-expression cassette of murine c-Myc, Klf4, Oct4 and Sox2 iPSC genes. Panel A. Gene Expression analysis of the 4-Factor PiggyBac Transposon Vector was tested in 3T3 Cells transfected with either no plasmid (-) or 2ug PiggyBac 4-in-1 vector and 0.5ug PiggyBac transposase (+). Cells were harvested at 2-weeks post-transfection, RNA was isolated and reverse transcribed (RT) to cDNA. Gene expression was evaluated via PCR using primers specific for 4-factors and Gapdh loading control. Panel B. 3T3 cells were visualized 2 weeks post-transfection of 2ug PiggyBac 4-in-1 transposon vector + 0.5ug PiggyBac Transposase for GFP expression and -/+ stained for anti-Sox2 antibody (Texas Red). DAPI stain was used as a positive control to identify cell nuclei.

Overexpress the microRNA miR-302bcad/367 cluster using PiggyBac

The PiggyBac Human microRNA miR-302bcad/367 iPSC Vectors. Choose from constitutive miR-302/367 expression (cat# PB-miR302) or inducible miR-302/367 expression via the cumate switch (cat# PBQM-miR302).

PiggyBac miR-302bcad/367 Cumate Switch Induction Data

The inducible PiggyBac vector with the miR-302bcad/367 microRNA cluster (cat# PBQM-miR302) features the ultra-tight cumate switch controlling the expression of the Human miR-302/367 microRNA cluster combined with the EF1-CymR repressor-T2A-Puro cassette to establish stable cell lines. Expression of your cDNA or microRNA of interest can be switched on simply by adding cumate to the cells. cat# PBQM-miR302 was co-transfected with the Super PiggyBac transposase (PB200PA-1) into 293T cells. The cells were allowed to successfully transpose the PBQM-miR302 transposon for 3 days. Induction was initiated by adding 30ug/ml cumate. Cells were harvetsed after 3 days and the microRNAs analyzed by qPCR with QuantiMir assays and normalizing to U6 levels to determine Fold Induction values.