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PiggyBac Transposon System

Instant and Reversible
Transgenesis

Cut and Paste Transposition

  • One transfection makes transgenic cell lines
  • Effective in Human, Mouse and Rat cells
  • No cargo limit — integrate 10-100kb
  • All-in-one tight inducible PiggyBac vector
  • PiggyBac copy number qPCR kit

The PiggyBac Transposon System

The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism. During transposition, the Super PB transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the PiggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes. The Super PB transposase (cat# PB210PA-1) is an engineered, hyperactive enzyme that catalyzes the integration of piggyBac transposons into TTAA sites in the target genome.

No cargo limit and can be re-excised

The unique features of PiggyBac transposons are that there is no cargo limit and it is also reversible. Genomes containing an inserted PiggyBac vector can be transiently transfected with the Excision only PB transposase (cat# PB220PA-1) expression vector to remove the transposons from the genome, footprint-free.

 

SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.