PiggyBac Transposon System
Instant and Reversible
Cut and Paste Transposition
- One transfection makes transgenic cell lines
- Effective in Human, Mouse and Rat cells
- No cargo limit — integrate 10-100kb
- All-in-one tight inducible PiggyBac vector
The PiggyBac Transposon System
The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the PiggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.
No cargo limit and can be re-excised
The unique features of PiggyBac transposons are that there is no cargo limit and it is also reversible. Genomes containing an inserted PiggyBac vector can be transiently re-transfected with the PB transposase expression vector. The PB transposase will remove the transposons from the genome, footprint-free.
SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.