The MycoQuick duplex PCR reaction

A positive control is included to control the successful amplification of PCR reaction and confirm the size of the PCR products in the testing samples. The mycoplasma primers will generate a 280 bp product. The UCE primers will generate a 205 bp band. The UCE primers confirm the absence of PCR inhibitors in all PCR samples. The UCE primers have been tested to detect an ultra-conserved element

Both mycoplasma primers and UCE primers are coated at the bottom of the each well of 96-well plate. Two amplifications can be achieved simultaneously in one PCR reaction. The two PCR products can be easily separated from each other on a 2.5% agarose gel.

MycoQuick kit format

The MycoQuick 96-well plate comes complete with mycoplasma primers and UCE primers coated at the bottom of each well. The 12 wells of the last lane also contain positive control DNA templates for mycoplasma and UCE. The plate can be cut into strips according to the number of the samples to be tested.

MycoQuick kit workflow