MetaMorph Mutagenesis Kit
Beautiful modifications made simple
Efficient and accurate mutagenesis
- Create single or multiple mutations simultaneously
- Introduce point mutations, deletions, or insertions
- No limit on the size of deletions or insertions
- Works with any vector
- Does not introduce unwanted mutations
Easy One-Day Protocol
Forward and Reverse Vector Primer Design
—The forward vector primer (in red) should be generated to the positive strand of DNA, and should contain ~20 bases corresponding to the vector sequence leading up to the restriction site where the gene-of-interest has been inserted.
—The reverse vector primer (in blue) should be generated to the negative strand of DNA, and should also contain ~20 bases complimentary to the vector sequence leading up to the restriction site where the gene-of-interest has been inserted.
Mutagenic Primer Design
—The reverse mutagenic primer (in red) should be generated to the negative strand of DNA, and should contain 10 bases 5' of the mutation, the mutagenic bases, and then 20 bases 3' of the mutation site.
—The forward mutagenic primer (in blue) should be generated to the positive strand of DNA, and should contain 10 bases 5' of the mutation, the mutagenic or inserted bases, and then 20 bases 3' of the mutation site.
—The forward and reverse mutagenic primers, therefore have a region of ~20 bases that is entirely complimentary and that contains the mutagenic bases.