miRNome MicroRNA Profilers

 

Easiest way to accurately profile microRNAs by qPCR

 

 

100% up-to-date with Sanger miRBase

 

 

 

 

These MicroRNA qPCR Array Panels come with all the reagents necessary to tag and convert small RNAs from 20 different total RNA samples into quantifiable cDNA using the sensitive QuantiMir™ technology. The kits include assays in pre-formatted plates for either 868 human or 627 mouse individual microRNAs with three endogenous reference RNA controls on each plate. All microRNA assays based on the Sanger miRBase microRNA database registered entries.

 

Features and Benefits

 

·  Real-time qPCR-based microRNA expression profiling system

·  SBI’s approach rapidly tags and converts all small RNAs into detectable cDNAs for qPCR – one cDNA synthesis required to quantitate our entire microRNA assay sets

·  Sensitive and quantitative down to picogram amounts of starting total RNA, start with as little 400 ng to profile all microRNAs

·  Three endogenous small RNA normalization genes included in panels (U6, RNU43 and U1).

·  Amenable to high-throughput analysis of numerous cell lines, tissues and clinical samples

·  Data analysis made easy with ΔΔCT calculation software included

 

Human miRNome Profiler

Mouse miRNome Profiler

·  All microRNAs, including all miR star* forms

·  Three 384-well plates of microRNA assays

·  Three endogenous controls on each plate: hsa-U6, RNU43 & U1

·  20 entire profiles in kit

·  All microRNAs, including all miR star* forms

·  Two 384-well plates of microRNA assays

·  Three endogenous controls on each plate: mmu-U6, RNU43 & U1

·  20 entire profiles in kit

Click here to view Human miRNome Profiler contents

Click here to view Mouse miRNome Profiler contents

 

What is a miR star* ?

 

miRNome microRNA Profiler Overview

 

miRNome microRNA

Profiler Workflow

 

What this Profiling

Array Kit does:

 

The miRNome microRNA Profilers enable the quantitation of all registered microRNAs along with 3 endogenous RNA controls for normalization.

 

1. QuantiMir cDNA technology tags and converts all small RNAs into cDNAs, ready to use as template for real-time qPCR.

 

   

 

2. Create mastermix with QuantiMir cDNA, 3’ universal reverse primer and 2X SYBR Green.  Pipet 5 μl into each well of a qPCR optical 384-well plate.

 

 

 

3. Pipet 1 μl of each of the microRNA assays from the miRNome assay stock plate into each well separately.

 

 

 

 

 

4. Perform Real-time qPCR runs using standard run conditions

(40 cycles, 60ºC anneal/extension) 

 

Kit comes with Data CD and ΔΔCT calculator

Text Box:  

 

  

Profile all microRNAs from 1 cDNA synthesis using as little as 400 ng of total RNA.

 

 

Kit Provides

User Provides

1. PolyA polymerase + Buffer solutions

2. Adaptor dT Primer

3. Reverse Transcriptase + Buffer solutions

4. Universal 3’ qPCR Primer

5. Primer set for MicroRNAs plus 3 controls on each plate

6. Complete kit for 20 entire profiles

7. CD with MicroRNA ID lists and simple relative quantitation spreadsheet calculator (ΔΔCt profiling)

1. RNA samples

2. SYBR green master mix

3. Optical qPCR plates

4. Real-time qPCR instrument

 

 

SBI has tested and recommends SYBR Green Master mix from three vendors: Power SYBR Master Mix® (Cat numbers 4368577, 4367650, 4367659, 4368706, 4368702, 4368708, 4367660) from Applied Biosystems; SYBR GreenER™ qPCR SuperMix for ABI PRISM® instrument from Invitrogen (Cat numbers 11760-100, 11760-500, and 11760-02K); and RT² Real-Time™ SYBR Green / ROX PCR (Cat numbers PA-012 and PA-112) from SuperArray.  For details of the buffer recommendation and experimental conditions, please see User Manual.

 

Endogenous Reference Controls

 

Technical Replicates

 

 

 

Kit Components

 

Optimized for 6 μl qPCR reactions

40

μl

5X PolyA polymerase Buffer

10

μl

PolyA polymerase

20

μl

25 mM MnCl2

30

μl

5 mM ATP

10

μl

3’ Oligo dT Adaptor

80

μl

5X Reverse Transcriptase Buffer

20

μl

Reverse Transcriptase

30

μl

0.1 M Dithiothreitol (DTT)

50

μl

dNTP Mix

2400

μl

3’ Universal Reverse PCR Primer

20

μl

Each of microRNA assays + three controls

1.2

ml

RNase-free Water

 

Kits should be stored at -20°C upon receipt.

 

User Manual

 

Ordering Information

 

Product Description

Catalog#

Size

Price

To Order

Human miRNome

microRNA Profiler

RA660A-1

20 profiles

$1,295

To Order

Mouse miRNome

microRNA Profiler

RA670A-1

20 profiles

$1,195

To Order

 

Custom Profiling Services

One week turnaround time!

 

Service Description

Catalog#

Size

Price

To Order

Human miRNome

microRNA Profiling Service

SVRA660A-1

1 RNA sample

$1,090

To Order

Mouse miRNome

microRNA Profiling Service

SVRA670A-1

1 RNA sample

$990

To Order

 

To obtain more information contact SBI at 888-266-5066 (toll-free in North America) or 650-968-2200, or e-mail to orders@systembio.com

 

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MicroRNA DiscoveryTM Kit