Sustained expression after transfection

Minicircles are easier to transfect into most cell types and expression can persist for weeks. Below is an example of transfection of 1 ug minicircle DNA of the pMC.CMV-MCS-EF1-GFP-SV40PolyA (catalog # MN511A-1) construct using standard transfection protocols.

Minicircle Hydrodynamic transfection through tail vein injection

Minicircle DNA can be transfected into animal models through tail vein injection procedures. Expression of the transgene cassette is sustained for many months where typical plasmid DNAs are silenced within hours. Hydrodynamic tail vein injection of 40 ug minicircle DNA results in a slight drop after 48 hours but then stabilizes over time for persistent expression. Mix 40 ug minicircle DNA expression DNA in saline equal to 10% of the rodent's body-weight (2 ml for a 20 g mouse). Inject into the tail vein rapidly (5-8 seconds).