MC-Easy™ Minicircle DNA production kit

The MC-Easy system enables the simple, reproducible and efficient way to produce high quality Minicircle DNA for your experiments. The finely tuned growth and induction media produce minicircle DNA that is free of parental and genomic DNA contamination. The Kit also includes an additional, innovative method for degrading any contaminating genomic DNA using an ATP-dependent DNase reagent that will selectively remove linear genomic and residual parental DNA but will not affect the minicircle DNA. Clean and effective minicircles every time. The system comes complete with a special minicircle DNA transfection reagent, MC-Fection, that works well with most cell lines. Choose from the minicircle production kit that includes the engineered E. coli producer strain ZYCY10P3S2T (cat# MN920A-1) or select the minicircle production and isolation kit alone (MN910A-1).

Kit components for catalog MN910A-1 and MN920A-1

• 5X Minicircle Growth medium (200 ml)
• 10X Induction Medium (100 ml)
• Minicircle DNA Re-purification kit (5 preps)
• Endotoxin free water (5 ml)
• E. coli producer strain ZYCY10P3S2T (included in cat# MN920A-1)

Download the MC-Easy Minicircle DNA Production System Protocol (PDF) »