MicroRNA Target
Selection System
Dual sensor system to identify
3' UTR targets
The Fire-Ctx vector
- Clone your 3'UTR
- Perform Ctx sensor screens
- Quantify binding using Luciferases
- Choose from catalog of full length 3'UTR clones
The MicroRNA Target Selection System
The miR-Selection lentivector features a dual reporter system with firefly luciferase (Fire) and a cytotoxic sensor (Ctx). The miR-Selection platform captures the 3’ UTR to microRNA binding event using a survival screen by modulating the reduction of the cytotoxic sensor. Quantitative validation is made simple using the built-in luciferase reporter. This powerful and elegant technology finally enables the accurate identification
of microRNA targets and enables high-throughput target identification screens.
Clone your 3'UTR into the vector—easily identify the microRNAs that bind
miR-Selection Lentivector details
• Total size of the lentivector = 10.2Kb
• Sequence of the Multiple Cloning Site (MCS) Starting with EcoRI and ending with NotI
5'-GAATTCGCTAGCTGAGGCAGCAGAGACCGGCCGGCCGGTCTCTCTCT-GTTCGAGGATCCCTCGAGGTCGACGCGGCCGC-3'