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MicroRNA Target
Selection System

Dual sensor system to identify
3' UTR targets

The Fire-Ctx vector

  • Clone your 3'UTR
  • Perform Ctx sensor screens
  • Quantify binding using Luciferases
  • Choose from catalog of full length 3'UTR clones

Online Resources

Product Citations

Nandita Sarkar, Fangting Wu, and Travis J. Antes. A novel cellular microRNA target selection system. BioTechniques, Vol. 48, No. 6, June 2010, pp. 466-467. (PDF) »

Technical References 

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Kaneda, R. and K. Fukuda, MicroRNA is a new diagnostic and therapeutic target for cardiovascular disease and regenerative medicine. Circ J, 2009. 73(8): p. 1397-8.

Hebert, S.S. and B. De Strooper, Alterations of the microRNA network cause neurodegenerative disease. Trends Neurosci, 2009. 32(4): p. 199-206. Min, H. and S. Yoon, Got target? Computational methods for microRNA target prediction and their extension. Exp Mol Med. 42(4): p. 233-44.

Du, L. and A. Pertsemlidis, microRNAs and lung cancer: tumors and 22-mers. Cancer Metastasis Rev. 29(1): p. 109-22.

Subramanian, S. and C.J. Steer, MicroRNAs as gatekeepers of apoptosis. J Cell Physiol. 223(2): p. 289-98.

Denli, A.M., et al., Processing of primary microRNAs by the Microprocessor complex. Nature, 2004. 432(7014): p. 231-5.

Yousef, M., L. Showe, and M. Showe, A study of microRNAs in silico and in vivo: bioinformatics approaches to microRNA discovery and target identification. FEBS J, 2009. 276(8): p. 2150-6.

Heikham, R. and R. Shankar, Flanking region sequence information to refine microRNA target predictions. J Biosci. 35(1): p. 105-18.

Sioud, M. and L. Cekaite, Profiling of miRNA expression and prediction of target genes. Methods Mol Biol. 629: p. 257-71.

Sachdeva, M., et al., p53 represses c-Myc through induction of the tumor suppressor miR-145. Proc Natl Acad Sci U S A, 2009. 106(9): p. 3207-12.

Sachdeva, M. and Y.Y. Mo, miR-145-mediated suppression of cell growth, invasion and metastasis. Am J Transl Res. 2(2): p. 170-80.