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SeraMir Exosome RNA Amplification

Exosome RNA Amplification and Profiling

Discover new serum biomarkers

  • Reduce variability by isolating exosomes first
  • Increased sensitivity by amplifying exoRNAs
  • Compatible with qPCR
  • Make amplified sense-strand exoRNAs for Microarrays and NextGen sequencing

How the SeraMir Kit Works

• Precipitate exosomes from patient biofluids easily with ExoQuick
• Purify exoRNAs with SeraMir columns
• Tail and tag all exoRNAs for qPCR, cDNA amplification and T7 IVT
• No RNA ligase used - avoid adaptor concatemer artifacts
• Amplify exoRNAs for Microarrays and NextGen Sequencing

Precipitate serum exosomes and purify exoRNAs

Exosome RNA Profiling

Tail exoRNAs and synthesize double-tagged cDNA

Exosome RNA Profiling