SeraMir Exosome RNA Amplification
Exosome RNA Amplification and Profiling
Discover new serum biomarkers
- Reduce variability by isolating exosomes first
- Increased sensitivity by amplifying exoRNAs
- Compatible with qPCR
- Make amplified sense-strand exoRNAs for Microarrays and NextGen sequencing
Better qPCR Profiling with SeraMir
Serum RNA prepared by conventional Trizol versus the SeraMir kit. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler (cat# RA810A-1). The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.
SeraMir Spike-in RNA control
Use the SeraMir spike-in RNA control and qPCR assay to control for exoRNA recovery, tailing and cDNA synthesis.
Human (cat# RA810A-) and Mouse (cat# RA811A-1) 384 SeraMir microRNA Profilers
Measure the top 380 microRNAs by qPCR across 20 exoRNA samples tagged using the SeraMir kit. The qPCR array also includes triplicate reactions for the RNA spike-in control.