MicroRNA Libraries
Screen for microRNA phenotypes
Pooled lentivirus libraries
- All microRNA clones represented
- Overexpress with Lenti-miRs
- Knockdown with miRZips
- Transduce all cell types, even stem cells
- Identify microRNA effectors using PCR
Perform one transduction and easily identify the microRNAs involved in your phenotypic screen
The Lenti-miR™ virus library contains a pool of SBI’s microRNA precursor clones and the miRZip™ virus library contains all of the miRZip anti-miR constructs in a ready-to-infect lentiviral preparation. The OncoMir virus library contains 141 of the most studied microRNAs involved in oncogenesis. Each virus within the Lenti-miR or OncoMir virus pool will express an individual microRNA precursor in its native context while preserving putative hairpin structures to ensure biologically relevant interactions with endogenous processing machinery and regulatory partners. Each virus in the miRZip library pool will express an individual anti-miR shRNA to permanently inhibit a specific microRNA. Details of the individual microRNA precursor clones and the list of the microRNAs which comprise the virus pool can be found on the Lenti-miR collection. The microRNA clones in the OncoMir virus library can be downloaded in the OncoMir virus Library List of Clones (PDF). More information on how miRZips work and the lists of the individual miRZip anti-miR clones in the virus pool can be found on the miRZip webpages.
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The microRNA or microRNAs responsible for generating the phenotypes of interest may be recovered through simple genomic PCR using the included lentivector-specific primers followed by direct sequencing. All microRNA precursor clones are about 500-700 bp in size and each miRZip shRNA segment amplified will be about 240 bp. |
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Quality Control of Lenti-miR Virus Library
Infectious titer is determined through real-time qPCR using SBI’s UltraRapid Lentiviral Titer Kit. Shown below is GFP expression in HT1080 cells transduced with the Lenti-miR Virus Library.
Lenti-miR Precursor Library Kit Components
- Aliquots of pooled Lenti-miR Virus Library (LVL)
- Tube of positive infection control virus (CD511VB-1)
- Tube of LVL PCR Primer Mix (10 μM of each primer)
- Each kit comes with user manual. PMIRHPLVA-1 (4 pooled virus aliquots: 5 to 10 screens)
PMIRHPLVAHT-1 (10 pooled virus aliquots: 12 to 40 screens)
miRZip anti-miR Library Kit Components
- One Aliquot of pooled miRZip Virus Library (MZIPPLVA)
- Tube of positive infection control virus for Scramble hairpin (MZIP000-VA-1)
- Tube of GNH PCR Primer Mix (10 μM of each primer)
- Each kit comes with user manual. MZIPPLVA-1 (1 pooled virus aliquot: 2 to 5 screens)