Pre-microRNA expression from constitutive CMV promoter
Reliable delivery to dividing or non-dividing cells
Monitor positive expressing cells with copGFP as a reporter under the control of a constitutive human elongation factor 1α (EF1) promoter, which is functional in most cell lines
Convenient sorting of transfected or transduced cells
Pool of prepackaged viral particles containing the entire microRNA Precursor Collection for high-throughput phenotypic screens also available