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miRZips anti-microRNAs

Permanent microRNA suppression

Permanent microRNA interference

  • Lentiviral-based anti-sense microRNAs
  • Use both GFP and Puro selection
  • Dissect microRNA regulated pathways

Online Resources

Product Citations

Mikhaylova O, Stratton Y, Hall D, Kellner E, Ehmer B, Drew AF, Gallo CA, Plas DR, Biesiada J, Meller J, Czyzyk-Krzeska MF. VHL-Regulated MiR-204 Suppresses Tumor Growth through Inhibition of LC3B-Mediated Autophagy in Renal Clear Cell Carcinoma. Cancer Cell. 2012 Apr 17;21(4):532-46.

Xin Liu, Yanli Wang, Qian Sun, Jie Yan, Jian Huang, Shiguo Zhu, Jianxiu Yu. Identification of microRNA transcriptome involved in human natural killer cell activation. Immunology Letters 143 (2012) 208-217. (PDF) »

Weeraratne SD, Amani V, Teider N, Pierre-Francois J, Winter D, Kye MJ, Sengupta S, Archer T, Remke M, Bai AH, Warren P, Pfister SM, Steen JA, Pomeroy SL, Cho YJ. Pleiotropic effects of miR-183~96~182 converge to regulate cell survival, proliferation and migration in medulloblastoma. Acta Neuropathol. 2012 Apr;123(4):539-52. (PDF) »

Liu S, Clouthier SG, Wicha MS. Role of microRNAs in the Regulation of Breast Cancer Stem Cells. J Mammary Gland Biol Neoplasia. 2012 Feb 14. [Epub ahead of print].

Cichocki F, Felices M, McCullar V, Presnell SR, Al-Attar A, Lutz CT, Miller JS. Cutting edge: microRNA-181 promotes human NK cell development by regulating Notch signaling. J Immunol. 2011 Dec 15;187(12):6171-5. (PDF) »

Smith AL, Iwanaga R, Drasin DJ, Micalizzi DS, Vartuli RL, Tan AC, Ford HL. The miR-106b-25 cluster targets Smad7, activates TGF-beta signaling, and induces EMT and tumor initiating cell characteristics downstream of Six1 in human breast cancer. Oncogene. 2012 Jan 30. doi: 10.1038/onc.2012.11. (PDF) »

Kane NM, Howard L, Descamps B, Meloni M, McClure JD, Lu R, McCahill A, Breen C, Mackenzie RM, Delles C, Mountford JC, Milligan G, Emanueli C, Baker AH. A Role for microRNAs 99b, 181a and 181b in the Differentiation to Vascular Endothelial Cells from Human Embryonic Stem Cells. Stem Cells. 2012 Jan 9. doi: 10.1002/stem.1026.

Png KJ, Halberg N, Yoshida M, Tavazoie SF. A microRNA regulon that mediates endothelial recruitment and metastasis by cancer cells. Nature. 2011 Dec 14. doi: 10.1038/nature10661. [Epub ahead of print].

Darido C, Georgy SR, Wilanowski T, Dworkin S, Auden A, Zhao Q, Rank G, Srivastava S, Finlay MJ, Papenfuss AT, Pandolfi PP, Pearson RB, Jane SM. Targeting of the Tumor Suppressor GRHL3 by a miR-21-Dependent Proto-Oncogenic Network Results in PTEN Loss and Tumorigenesis. Cancer Cell. 2011 Nov 15;20(5):635-48. (PDF) »

Fu J-D, Rushing SN, Lieu DK, Chan CW, Kong C-W, et al. Distinct Roles of MicroRNA-1 and -499 in Ventricular Specification and Functional Maturation of Human Embryonic Stem Cell-Derived Cardiomyocytes. PLoS ONE 6(11): e27417. doi:10.1371/journal.pone.0027417. (PDF) »

Li N, Kaur S, Greshock J, Lassus H, Zhong X, Wang Y, Leminen A, Shao Z, Hu X, Liang S, Katsaros D, Huang Q, Butzow R, Weber BL, Coukos G Sr, Zhang L. A combined array-based comparative genomic hybridization (aCGH) and functional library screening approach identifies mir-30d as an oncomir in cancer. Cancer Res. 2011 Nov 4. [Epub ahead of print].

Guibinga GH, Hrustanovic G, Bouic K, Jinnah HA, Friedmann T. MICRORNA-MEDIATED DYSREGULATION OF NEURAL DEVELOPMENTAL GENES IN HPRT DEFICIENCY: CLUES FOR LESCH NYHAN DISEASE? Hum Mol Genet. 2011 Oct 31. [Epub ahead of print].

Mitra D, Das PM, Huynh FC, Jones FE. Jumonji/Arid1 B (JARID1B) promotes breast tumor cell cycle progression through epigenetic repression of micro RNA let-7e. AJ Biol Chem. 2011 Oct 3. [Epub ahead of print]. (PDF) »

Shtutman M, Baig M, Levina E, Hurteau G, Lim CU, Broude E, Nikiforov M, Harkins TT, Carmack CS, Ding Y, Wieland F, Buttyan R, Roninson IB. Tumor-specific silencing of COPZ2 gene encoding coatomer protein complex subunit Zeta-2 renders tumor cells dependent on its paralogous gene COPZ1. Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12449-54.

Kojima K, Takata A, Vadnais C, Otsuka M, Yoshikawa T, Akanuma M, Kondo Y, Kang YJ, Kishikawa T, Kato N, Xie Z, Zhang WJ, Yoshida H, Omata M, Nepveu A, Koike K. MicroRNA122 is a key regulator of alpha-fetoprotein expression and influences the aggressiveness of hepatocellular carcinoma. Nat Commun. 2011 Jun 7;2:338. (PDF) »

Ghisi M, Corradin A, Basso K, Frasson C, Serafin V, Mukherjee S, Mussolin L, Ruggero K, Bonanno L, Guffanti A, De Bellis G, Gerosa G, Stellin G, D'Agostino DM, Basso G, Bronte V, Indraccolo S, Amadori A, Zanovello P. Modulation of microRNA expression in human T-cell development: targeting of NOTCH3 by miR-150. Blood. 2011 Jun 30;117(26):7053-62.

Imane Azzouzi, Hansjoerg Moest, Jeannine Winkler, Jean-Claude Fauchere, Andre P. Gerber, Bernd Wollscheid, Markus Stoffel, Markus Schmugge, Oliver Speer. MicroRNA-96 Directly Inhibits Gamma-Globin Expression in Human Erythropoiesis. PLoS ONE 6(7): e22838. doi:10.1371/journal.pone.0022838. (PDF) »

Fu Yang,Ling Zhang,Fang Wang, Yue Wang, Xi-song Huo, Yi-xuan Yin, Yu-qi Wang, Lin Zhang, and Shu-han Sun. Modulation of the Unfolded Protein Response Is the Core of MicroRNA-122-Involved Sensitivity to Chemotherapy in Hepatocellular Carcinoma. Neoplasia. 2011 July; 13(7): 590-600.

Mavrakis KJ, Van Der Meulen J, Wolfe AL, Liu X, Mets E, Taghon T, Khan AA, Setti M, Rondou P, Vandenberghe P, Delabesse E, Benoit Y, Socci NB, Leslie CS, Van Vlierberghe P, Speleman F, Wendel HG. A cooperative microRNA-tumor suppressor gene network in acute T-cell lymphoblastic leukemia (T-ALL). Nat Genet. 2011 Jun 5. [Epub ahead of print].

Stephanie Ma, Yuen Piu Chan, Pak Shing Kwan, Terence K. Lee, Mingxia Yan, Kwan Ho Tang, Ming Tat Ling, Juergen R. Vielkind, Xin-Yuan Guan, and Kwok Wah Chan. MicroRNA-616 Induces Androgen-Independent Growth of Prostate Cancer Cells by Suppressing Expression of Tissue Factor Pathway Inhibitor TFPI-2. Cancer Res. 2011 Jan 15;71(2):583-92. (PDF) »

Katey J. Rayner, Yajaira Suárez, Alberto Dávalos, Saj Parathath, Michael L. Fitzgerald, Norimasa Tamehiro, Edward A. Fisher, Kathryn J. Moore, Carlos Fernández-Hernando. miR-33 Contributes to the Regulation of Cholesterol Homeostasis. Science. 2010 Jun 18;328(5985):1570-3.

Su X, Chakravarti D, Cho MS, Liu L, Gi YJ, Lin YL, Leung ML, El-Naggar A, Creighton CJ, Suraokar MB, Wistuba I, Flores ER. TAp63 suppresses metastasis through coordinate regulation of Dicer and miRNAs. Nature. 2010 Oct 21;467(7318):986-90.

Hassan MQ, Gordon JA, Beloti MM, Croce CM, van Wijnen AJ, Stein JL, Stein GS, Lian JB. A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program. Proc Natl Acad Sci USA. 2010 Oct 27. [Epub ahead of print]. (PDF) »

Xia H, Cheung WK, Sze J, Lu G, Jiang S, Yao H, Bian XW, Poon WS, Kung HF, Lin MC. miR-200a regulates epithelial-mesenchymal to stem-like transition via ZEB2 and beta-catenin signaling. J Biol Chem. 2010 Nov 19;285(47):36995-7004. (PDF) »

Wilson KD, Hu S, Venkatasubrahmanyam S, Fu JD, Sun N, Abilez OJ, Baugh JJ, Jia F, Ghosh Z, Li RA, Butte AJ, Wu JC. Dynamic MicroRNA Expression Programs during Cardiac Differentiation of Human Embryonic Stem Cells: Role for miR-499. Circ Cardiovasc Genet. 2010 Aug 23. [Epub ahead of print].

Konstantinos J. Mavrakis, Andrew L. Wolfe, Elisa Oricchio, Teresa Palomero, Kim de Keersmaecker, Katherine McJunkin, Johannes Zuber, Taneisha James, Aly A. Khan, Christina S. Leslie, Joel S. Parker, Patrick J. Paddison, Wayne Tam, Adolfo Ferrando & Hans-Guido Wendel. Genome-wide RNA-mediated interference screen identifies miR-19 targets in Notch-induced T-cell acute lymphoblastic leukaemia. Nature Cell Biology 12, 372 - 379 (2010).

Li QQ, Chen ZQ, Cao XX, Xu JD, Xu JW, Chen YY, Wang WJ, Chen Q, Tang F, Liu XP, Xu ZD. Involvement of NF-kappaB/miR-448 regulatory feedback loop in chemotherapy-induced epithelial-mesenchymal transition of breast cancer cells. Cell Death Differ. 2010 Aug 27. [Epub ahead of print].

Angelo Veronese, Laura Lupini, Jessica Consiglio, Rosa Visone, Manuela Ferracin, Francesca Fornari, Nicola Zanesi, Hansjuerg Alder, Gemma D'Elia, Laura Gramantieri, Luigi Bolondi, Giovanni Lanza, Patrizia Querzoli, Adriano Angioni, Carlo M. Croce, and Massimo Negrini. Oncogenic Role of miR-483-3p at the IGF2/483 Locus. Cancer Research 70, 3140, April 15, 2010. (PDF) »

Lovén J, Zinin N, Wahlström T, Müller I, Brodin P, Fredlund E, Ribacke U, Pivarcsi A, Páhlman S, Henriksson M. MYCN-regulated microRNAs repress estrogen receptor-alpha (ESR1) expression and neuronal differentiation in human neuroblastoma. Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1553-8. (PDF) »

Hasan Rajabi, Caining Jin, Rehan Ahmad, Andrew Cain McClary, Maya Datt Joshi and Donald Kufe. Mucin 1 Oncoprotein Expression Is Suppressed by the miR-125b Oncomir. Genes and Cancer Jan 2010, vol. 1 no. 1 62-68. (PDF) »

Greene SB, Gunaratne PH, Hammond SM, Rosen JM. A putative role for microRNA-205 in mammary epithelial cell progenitors. J Cell Sci. 2010 Feb 15;123(Pt 4):606-618. (PDF) »