Detect microRNAs involved with cellular differentiation
microRNAs (miRNAs) are an abundant class of endogenous non-protein-coding small RNAs, which negatively regulate gene expression at the posttranscriptional level in many developmental and metabolic processes. miRNAs regulate a variety of biological processes, including developmental timing, signal transduction, tissue differentiation and maintenance, disease, and carcinogenesis. Emerging evidence demonstrates that miRNAs also play an essential role in stem cell self-renewal and differentiation. Some miRNAs are specifically expressed in stem cells, control stem cell self-renewal, and differentiation through negatively regulating the expression of certain key genes in stem cells.
The microRNA qPCR assays on the Stem Cell panel are a collection of microRNAs involved in Stem Cell self-renewal, hematopoiesis, neural development and tissue patterning.
Features and Benefits
Simultaneously profile 95 different MicroRNAs known to be involved in Stem Cell self-renewal, hematopoiesis, neural development and tissue patterning
Identify MicroRNA development biomarkers and expression pattern signatures in Stem Cells
System rapidly tags and converts all small RNAs into detectable cDNA for qPCR
Simple and robust procedure
Sensitive and quantitative down to picogram amounts of starting total RNA
Amenable to high-throughput screening of numerous cell lines and tissues
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MicroRNA expression profiling during human cord blood-derived CD34 cell erythropoiesis. Exp Hematol. 2007 Apr;35(4):551-64. Choong ML, Yang HH, McNiece I.
MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Res. 2006 Jan 24;34(2):e9. Tang F, Hajkova P, Barton SC, Lao K, Surani MA.
Stem cells: from epigenetics to microRNAs. Neuron. 2005 May 5;46(3):363-7. Review. Cheng LC, Tavazoie M, Doetsch F.
MicroRNA: a new player in stem cells. J Cell Physiol. 2006 Nov;209(2):266-9. Review. Zhang B, Pan X, Anderson TA.
Human embryonic stem cells express a unique set of microRNAs. Dev Biol. 2004 Jun 15;270(2):488-98. Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, Cha KY, Chung HM, Yoon HS, Moon SY, Kim VN, Kim KS.
