OncoMir Collection
Profile microRNAs in Cancer
Pre-formatted qPCR array
- Oncogenic markers
- Tumor progressive microRNAs
- Anti-apoptotic microRNAs
Features and Benefits
- Simultaneously profile 95 different MicroRNAs known to be involved in apoptosis, differentiation and Cancer
- Identify MicroRNA biomarkers and expression pattern signatures
- System rapidly tags and converts all small RNAs into detectable cDNA for qPCR
- Simple and robust procedure
- Sensitive and quantitative down to picogram amounts of starting total RNA
- Amenable to high-throughput screening of clinical samples including biopsies and LCM samples
OncoMir microRNA qPCR Array Layout
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | let-7 family |
miR- 7 |
miR-
92a |
miR- 93 |
miR- 9 |
miR- 101 |
miR-
103 |
miR- 106a |
miR- 106b |
miR- 107 |
miR- 10b |
miR- 1 |
| B | miR- 122 |
miR- 125a-5p |
miR- 125b |
miR- 126 |
miR- 128 |
miR- 132 |
miR- 133a |
miR- 134 |
miR- 135b |
miR- 136 |
miR- 137 |
miR- 140 |
| C | miR- 141 |
miR- 142-3p |
miR- 143 |
miR- 145 |
miR- 146a |
miR- 149 |
miR- 150 |
miR- 151 |
miR- 153 |
miR- 154 |
miR- 155 |
miR- 15a |
| D | miR- 15b |
miR- 16 |
miR- 17* |
miR- 17 |
miR- 181a |
miR- 181b |
miR- 181c |
miR- 181d |
miR- 183 |
miR- 185 |
miR- 186 |
miR- 188 |
| E | miR- 18a |
miR- 190 |
miR- 191 |
miR- 192 |
miR- 194 |
miR- 195 |
miR- 196a |
miR- 197 |
miR- 198 |
miR- 199ab |
miR- 30b |
miR- 19ab |
| F | miR- 95 |
miR- 20a |
miR- 200a |
miR- 200b |
miR- 200c |
miR- 202 |
miR- 203 |
miR- 204 |
miR- 205 |
miR- 206 |
miR- 21 |
miR- 210 |
| G | miR- 214 |
miR- 215 |
miR- 372 |
miR- 373 |
miR- 218 |
miR- 219 |
miR- 22 |
miR- 488 |
miR- 221 |
miR- 222 |
miR- 223 |
miR- 224 |
| H | miR- 23a |
miR- 24 |
miR- 25 |
miR- 26a |
miR- 26b |
miR- 27ab |
miR- 30c |
miR- 29abc |
miR- 30a* |
miR- 30a |
miR- 296 |
U6 snRNA |
Sample MicroRNA qPCR Array Data
User-generated qPCR Data from Human clinical biosies from Normal and Tumor tissue.
Kit Components
|
40µl
|
5x PolyA polymerase Buffer |
|
10µl
|
PolyA polymerase |
|
20µl
|
25 mM MnCl2 |
|
30µl
|
5 mM ATP |
|
10µl
|
3' Oligo dT Adaptor |
|
80µl
|
5x Reverse Transcriptase Buffer |
|
20µl
|
Reverse Transcriptase |
|
30µl
|
0.1 M Dithiothreitol (DTT) |
|
50µl
|
dNTP Mix |
|
600µl
|
3' Universal Reverse PCR Primer |
+ One 96-well Cancer microRNA Primer stock plate (enough for 10 entire panel measurements)
Kits should be stored at -20oC upon receipt.