HT Genome Wide Assays

miRNome MicroRNA Profilers

Genome-wide qPCR microRNA expression profiling

 

These MicroRNA qPCR Array Panels come with all the reagents necessary to tag and convert small RNAs from 20 different total RNA samples into quantifiable cDNA using the sensitive QuantiMir™ technology.

The kits include assays in pre-formatted plates for either the full compliment of human, mouse or rat individual microRNAs with three endogenous reference RNA controls on each plate (see Table below). All microRNA assays are based on the Sanger miRBase microRNA database registered entries.

Sanger miRBase (http://www.mirbase.org/search.shtml).

The most comprehensive qPCR arrays available only from SBI

Features and Benefits

Human miRNome Profiler	Mouse miRNome Profiler
All microRNAs, including all miR star* forms Three 384-well plates of microRNA assays Three endogenous controls on each plate: hsa-U6, RNU43 & U1 20 entire profiles in kit	All microRNAs, including all miR star* forms Two 384-well plates of microRNA assays Three endogenous controls on each plate: mmu-U6, RNU43 & U1 20 entire profiles in kit

Rat miRNome Profiler

All microRNAs, including all miR star* forms Two 384-well plate of microRNA assays Three endogenous controls on each plate: rno-U6, RNU43 & U1 20 entire profiles in kit

miRNome Profiler Analysis, Sanger ver. 14

Three validated reference controls included on each qPCR array plate

Accurately normalize your microRNA expression profiling data using three reference controls for endogenous housekeeping small RNA controls. Included on each plate are QuantiMir assays for RNUA1 (U1 snRNA), RNU43 (snoRNA-43) and RNU6 (U6 snRNA). The data anlaysis software calculates the geometric mean of all three signals prior to normalizing the qPCR microRNA profiling data. The values calculated reflect the ΔΔCt expression measurements and are automatically graphed. More information for the endogenous reference controls is located in the microRNA resources section.

Excellent technical replicates across 8 logs

Reliable replicates from plate-to-plate enable the precise comparisons from numerous samples across large microRNA profiling studies.

Online Resources

Product Citations

Nicole Noren Hooten, Kotb Abdelmohsen, Myriam Gorospe, Ngozi Ejiogu, Alan B. Zonderman, Michele K. Evans. MicroRNA Expression Patterns Reveal Differential Expression of Target Genes with Age. 2010 PLoS ONE 5(5): e10724. doi:10.1371/journal.pone.0010724. (PDF) »

Marasa BS, Srikantan S, Martindale JL, Kim MM, Lee EK, Gorospe M, Abdelmohsen K. MicroRNA profiling in human diploid fibroblasts uncovers miR-519 role in replicative senescence. Aging 2010 Jun;2(6):333-43. (PDF) »

Juan D, Alexe G, Antes T, Liu H, Madabhushi A, Delisi C, Ganesan S, Bhanot G, Liou LS. Identification of a MicroRNA Panel for Clear-cell Kidney Cancer. Urology 1-7. 2009 Dec 24. (PDF) »

MicroRNA Technical References and Reviews

Cancer genomics: small RNAs with big impacts.
Nature. 2005 Jun 9;435(7043):745-6.
Meltzer, PS.

MicroRNAs in Gene Regulation: When the Smallest Governs It All.
J Biomed Biotechnol. 2006;2006(4):69616.
Ouellet DL, Perron MP, Gobeil LA, Plante P, Provost P.

MicroRNAs as oncogenes.
Curr Opin Genet Dev. 2006 Feb;16(1):4-9. Epub 2005 Dec 19.
Hammond SM.

Oncomirs - microRNAs with a role in cancer.
Nat Rev Cancer. 2006 Apr;6(4):259-69.
Esquela-Kerscher A, Slack FJ.

microRNAs as oncogenes and tumor suppressors.
Dev Biol. 2006 Aug 16; [Epub ahead of print]
Zhang B, Pan X, Cobb GP, Anderson TA.

MicroRNAs and the hallmarks of cancer.
Oncogene. 2006 Oct 9;25(46):6170-5.
Dalmay T, Edwards DR

microRNAs exhibit high frequency genomic alterations in human cancer.
Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9136-41. Epub 2006 Jun 5.
Zhang L, Huang J, Yang N, Greshock J, Megraw MS, Giannakakis A, Liang S, Naylor TL, Barchetti A, Ward MR, Yao G, Medina A, O'brien-Jenkins A, Katsaros D, Hatzigeorgiou A, Gimotty PA, Weber BL, Coukos G.

miRNome MicroRNA qPCR Profilers Quantity Product Size Catalog # Price hsa-miRNome MicroRNA Profiling Kit [Human] 20 complete profiles RA660A-1 $1,359.75 mmu-miRNome MicroRNA Profiling Kit [Mouse] 20 complete profiles RA670A-1 $1,254.75 rno-miRNome MicroRNA Profiling Kit [Rat] 20 complete profiles RA680A-1 $875.75 All miRNome Profiler qPCR array kits come complete with enough microRNA assays for 20 genome-wide profiles and three endogenous reference control assays per qPCR array plate. The qPCR arrays are provided in 384-well format. miRNome MicroRNA Profiling Service Quantity Product Size Catalog # Price Human miRNome Profiling Custom Service 1 sample SVRA660A-1 $1,090.00 Mouse miRNome Profiling Custom Service 1 sample SVRA670A-1 $990.00 Rat miRNome Profiling Custom Service 1 sample SVRA680A-1 $790.00

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