Accurate and sensitive qPCR systems for microRNA detection
MicroRNA and siRNA expression analysis is made easy with the QuantiMir™ RT systems. QuantiMir is used to generate PCR-ready cDNA from total RNA in a robust manner which is much more sensitive, quantitative and easier than Northerns or RNase protection experiments. Synthesize enough cDNA template for multiple measurements from a single RT reaction.
This technology is also available combined with pre-formatted microRNA qPCR assay panels for Cancer microRNAs and for Stem Cell differentiation-associated microRNAs. SBI also offers genome-wide microRNA expression profiling qPCR assay sets and services.
How sensitive is the QuantiMir microRNA profiling platform?
The starting total RNA general guideline is 100 pg total RNA per microRNA you want to quantitate. Example: Start with 10 ng total RNA = Measure 100 microRNAs