Exosome antibodies for Western blot analysis

For Western blotting analysis, we recommend resuspending the exosome pellet in 1XRIPA buffer with the appropriate protease inhibitor cocktail. SBI offers individual antibodies for CD63, CD9, CD81 and Hsp70 as well as a Western blot sampler kit (Catalog# EXOAB-KIT-1) which includes four exosomal marker antibodies: CD63, CD9, CD81, HSP70 (rabbit anti-human) and also includes a goat anti-Rabbit IgG HRP conjugated secondary antibody specifically tested for use in exosomal protein analysis. The primary antibodies are used at a 1:1,000 dilution and the HRP secondary antibody at 1:20,000 dilution.

Exosome Marker Protein Analysis