ExoQuick™ and ExoQuick-TC™
Polymer-based exosome precipitation
One-step Exosome Isolation
- Serum and Plasma
- Tumor Ascites Fluid
- Urine
- Spinal fluid
- Tissue Culture Media
Analysis of ExoQuick Serum and Urine exosomes using the NanoSight LM10
The NanoSight LM10 instrument is based on a conventional optical microscope and uses a laser light source to illuminate nano-scale particles within a 0.3 ml sample introduced to the viewing unit with a disposable syringe. Enhanced by a near perfect black background, particles appear individually as point-scatterers moving under Brownian motion.
The image analysis Nanoparticle Tracking Analysis (NTA) software suite allows users to automatically track and size nanoparticles on an individual basis. Results are displayed as a frequency size distribution graph and output to spreadsheet. In addition, video clips of images may be captured and archived for future reference.
ExoQuick serum exosome analysisNormal human serum from 50 pooled samples was used. Only 250ul serum was combined with 63ul ExoQuick to pellet the exosomes in 30 minutes. The exosome pellet was resuspended in 100ul PBS, diluted 1:10,000 and visualized on the NanoSight LM10 instrument. The analysis shows that ExoQuick isolated 90nm exosomes with a recovery of 2.74 x 10^12 particles/ml. |
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ExoQuick-TC conditioned media exosome analysisHuman HT1080 lung sarcoma cell line was cultured in conditioned media (serum-free) for 72 hours. Ten milliliters of the media was combined with 2ml ExoQuick-TC to pellet the exosomes overnight. The exosome pellet was resuspended in 1ml PBS, diluted 1:40 and visualized on the NanoSight LM10 instrument. The analysis shows that ExoQuick isolated 133nm exosomes with a recovery of 1.74 x 10^9 particles/ml. |
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Data acquiistion and analysis
These experiments were done in collaboration with Particle Characterization Laboratories, Inc (PCL). For more information on PCL and their particle analysis services, click here.
