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Size Distribution of Amplified cDNA
Global amplification of small RNAs. The
size distribution and banding pattern of amplified cDNA is shown in Figure 2.
In this study, 50 ng of a pool of RNAs from 4 different tissues was amplified with the SBI kit and a
small portion examined by gel electrophoresis. The results are shown in Figure 2.
Figure 2. Size distribution of amplified cDNA
A banding pattern was observed which is consistent with amplification of
several highly abundant small RNAs, as indicated. Because of the very small
size and heterogeneity of microRNAs (19-24 nucleotides), and low abundance,
it is not possible to observe the microRNA. However, as shown in
Figure 1,
the presence of microRNA is clearly visible. The yield of amplified cDNA is
sufficient for several hundred quantitative PCR experiments or several
microRNA microarray experiments. Note that the sizes of the microRNA
amplified species (including the 60 nt adaptors) would range from 79 to 84
nts.
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