|
Good Correlation Between Amplified and
Non-Amplified RNA
For any method of gene transcript
amplification method, it is important that the relative levels of RNAs be
maintained during the amplification process. A quantitative comparison of
the levels of small RNA before and after amplification is shown in Fig 3. In
this experiment, 50 ng of a pool of RNAs from 4 different
tissues was amplified with the SBI kit and tested for the presence of 10
different noncoding small RNA by RT-PCR.
The same RNA pool was tested and quantitated for the same small noncoding
RNA before amplification. Care was taken to avoid the plateau phase of the
PCR amplification.
The results are shown in Fig. 3. For all 10 small RNAs, the relative levels
were maintained before and after amplification.
Figure 3. Maintenance of relative
expression levels of RNA amplified using the SBI kit.
|
