Profile Human lncRNAs in cancer

Comparison of lncRNA expression in Human normal Fibroblasts and Fibrosarcoma cells
Total RNA was isolated from approximately 2x10^6 cells using standard Trizol/Ethanol precipitation methods. Two micrograms of the RNA samples were used as input into the lncRNA cDNA synthesis kit. The entire cDNA synthesized was then profiled across the 90 lncRNAs and 5 reference controls on the LncProfiler qPCR array. LncRNAs known to be upregulated in cancer, such as Neat1, Anril, Hotair and Malat1 were all detected at highly elevated levels in the fibrosarcoma cells (HT1080) when compared to the normal fibroblast cells (HFF). The data are plotted as normalized expression levels. Selected data are shown in the bar graph.

Profile Mouse lncRNA differential tissue expression patterns

Profiling Mouse lncRNA expression profiles across various tissues Two micrograms of the various tissue RNA samples were used as input into the lncRNA cDNA synthesis kit. The entire cDNA synthesized was then profiled across the 90 lncRNAs and 5 reference controls on the Mouse LncProfiler qPCR array. Mouse lncRNAs are differentially expressed across the tissue sets. Representative data are shown in the bar graph.