• Efficient delivery:
  System created by nature for efficient and robust delivery of genetic material to most cell types, including non-dividing and hard-to-transfect cells (primary, blood, stem cells) in vitro or to transgenic mice models in vivo
   

• Stable, heritable expression:
  Constructs integrated into genomic DNA--high expression levels in chromosomal environment, with low variability
   

• Non-disruptive to cells:
  Cell functions are unaffected by the infection process
   

• Safe:
  Replication incompetent design Choice of HIV and non-human FIV vectors

SBI offers an extended set of cloning and expression lentivectors for efficient delivery of expression constructs in mammalian systems. To achieve highly efficient delivery and stable expression, the lentiviral constructs can be packaged in VSV-G pseudoviral particles and transduced into a wide range of cell lines or to model organisms (mouse, rat, etc.).

In comparison with traditional retroviral delivery systems, lentivectors actively enter the cell nucleus without requiring cell replication. SBI offers lentivectors derived from commonly-used HIV (human immunodeficiency virus) and FIV (feline immunodeficiency virus). Both types of lentivectors have similar structures and performance, but may have different transduction efficiencies for different cell types. Although higher titers are usually achieved with HIV-based vectors, FIV-based vectors are considered a biologically-safe alternative as they are derived from a non-human feline virus.

SBI’s Lentivector Expression System provides you with the tools to conveniently and reliably…

(1) Generate lentivector constructs.

(2) Package the viral-vector expression constructs into VSV-G pseudotyped viral particles.

(3) Use the packaged pseudoviral particles to introduce and stably express your construct in almost any mammalian system—including hard-to-transfect systems, such as primary cells, neuronal cells, lymphocytes, etc.

Lentiviral System Products
pPACK Lentivector Packaging System
Packaged Positive Transduction Control
 
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