SBI’s pGreenFire lentivectors enable the simultaneous tracking of GFP and the quantitative measurement of Luciferase reporters.

Sample data using the GreenFire dual reporters

Sample Data using the pGreenFire1-NF-κB Transcription Reporter

Monitoring NF−κB transactivation using GFP epifluorescence.

293TN cells were transduced with the packaged pGF1-NFκB reporter virus. Two days later, the cells were split into 96 well plates and then treated with the indicated amount of TNF-α. Levels of NFκB activation was assessed 18 hours post-induction using epifluorescence microscopy.

Quantitative analysis of NF-κB transactivation using Firefly Luciferase

293TN cells were transduced with either the packaged pGF1-NFκB or pGF1-mCMV (negative control) reporter virus. Two days later, the cells were split into 96 well plates and then treated with the indicated amount of TNF-α. Levels of NFκB activation was quantitated 18 hours post-induction using a standard firefly luciferase assay (Promega).