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pGreenFire™ Pathway Reporters

Lenti-based monitors of transcription activation

Dual Reporter Lentivector Systems

  • Track postive cells with GFP
  • Quantify level of transactivation with Luciferase
  • Ready-to-transduce virus

Online Resources

Product Citations

Singh BN, Fu J, Srivastava RK, Shankar S. Hedgehog Signaling Antagonist GDC-0449 (Vismodegib) Inhibits Pancreatic Cancer Stem Cell Characteristics: Molecular Mechanisms. PLoS One. 2011;6(11):e27306. (PDF) »

Fishel ML, Jiang Y, Rajeshkumar NV, Scandura G, Sinn AL, He Y, Shen C, Jones DR, Pollok KE, Ivan M, Maitra A, Kelley MR. Impact of APE1/Ref-1 redox inhibition on pancreatic tumor growth. Mol Cancer Ther. 2011 Sep;10(9):1698-708.

Jabbour AM, Daunt CP, Green BD, Vogel S, Gordon L, Lee RS, Silke N, Pearson RB, Vandenberg CJ, Kelly PN, Nutt SL, Strasser A, Borner C, Ekert PG. Myeloid progenitor cells lacking p53 exhibit delayed up-regulation of Puma and prolonged survival after cytokine deprivation. Blood. 2010 Jan 14;115(2):344-52.

Nassirpour R, Shao L, Flanagan P, Abrams T, Jallal B, Smeal T, Yin MJ. Nek6 mediates human cancer cell transformation and is a potential cancer therapeutic target. Mol Cancer Res. 2010 May;8(5):717-28.

Emilia Alina Barbu, Juan Zhang, and Reuben P. Siraganian. The Limited Contribution of Fyn and Gab2 to the High Affinity IgE Receptor Signaling in Mast Cells. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 285, NO. 21, pp. 15761—15768, May 21, 2010. (PDF) »

Yong Pil Hwang a, Hyo Jeong Yun a,b, Hyung Gyun Kim a, Eun Hee Han a, Gye Won Lee c, Hye Gwang. Suppression of PMA-induced tumor cell invasion by dihydroartemisinin via inhibition of PKCa/Raf/MAPKs and NF-kB/AP-1-dependent mechanisms. Biochemical Pharmacology 79 (2010) 1714—1726. (PDF) »

Christina H. Stuelten, Anil K. Kamaraju, Lalage M.Wakefield and Anita B. Roberts. Lentiviral reporter constructs for fluorescence tracking of the temporospatial pattern of Smad3 signaling. BioTechniques 43:289-294 (September 2007). (PDF) »

Ebauer M., Wachtel M., Niggli F. K., Schafer B. W. Comparative expression profiling identifies an invi target gene signature with TFAP2B as a mediator of the survival function of PAX3/FKHR. Oncogene Advance Online Publication May 21, 2007; doi: 10.1038/sj.onc.1210525. (PDF) »

Technical references

Poeschla EM, Wong-Staal F, Looney DJ. Efficient transduction of nondividing human cells by feline immunodeficiency virus lentiviral vectors. Nat Med. 1998 Mar; 4(3): 354-7.

Poeschla, E.M., Looney, D.J., and Wong-Staal, F. (2003) Lentiviral nucleic acids and uses thereof. US Patent NO. 6,555,107 B2.

Dull, T., Zufferey, R., Kelly, M., Mandel, R.J., Nguyen, M, Trono, D. (1998) J. Virol.,72, 8463-8471.

Miyoshi, H., Blomer, U., Takashi, M., Gage, F.N., Verma, I.M (1998), J.Virol., 72, 8150-8157.

Zufferey, R., Donello, J.E., Trono, D., Hope, T.J. (1999), J.Virol., 73, 2886-2892.

Ramezani, A., Hawley, T.S., Hawley, R.G. (2000) Mol. Ther., 2, 458-469.

Leung, T.H., Hoffmann, A., Baltimore, D. 2004, Cell, v. 118, 453-464.