NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line

Monoclonal human embryonic kidney (HEK)-293-based cell line for analysis of Nuclear Factor kappa B (NF-κB) pathway activation featuring GFP and Luciferase dual reporter system (cat# TR860A-1).

Highly sensitive reporter cell line - first of its kind with Dual reporters for GFP and Firefly Luciferase
Monoclonal HEK-293 cell line with stably integrated lentiviral transcriptional reporter vector with 300-fold NF-κB-dependent activation
Monitor transactivation in live cells with GFP
Quantitate NF-κB response using Luciferase

The NF-κB/293/GFP-Luc™ cell line was specifically designed for monitoring the NF-κB signal transduction pathway in vitro. The unique features of this reporter cell line allows researchers to monitor the NF-κB pathway by the detection of GFP fluorescence as well as Luciferase for quantitative transcription activation reporter assays. The NF-κB/293/GFP™ cells were derived from System Biosciences’ 293 TN Producer Cell Line (Cat. # LV900A-1). 293 TN cells were transduced with HIV-based pseudoviral particles packaged with a lentivector that co-expresses destabilized copGFP and Firefly Luciferase reporters coupled with the minimal cytomegalovirus (mCMV) promoter in conjunction with four copies of the NF-κB consensus transcriptional response elements.

Monitor NF-κB Activation in real-time with GFP

Quantitate NF-κB Activation with Luciferase Assays

Highly sensitive cell line responds to TNFα as seen with the GFP FACS data above. The GFP fluorescence saturates and the Luciferase quantitation assays clearly reveal a linear induction relationship with increasing amounts of TNFα.

NF-κB

NF-κB, a member of the rel family of transcription factors, regulates several important physiological processes, including immune responses, inflammation, cell growth, apoptosis, tumorigenesis, and the expression of certain virus genes (HIV and CMV). As a result, the NF-κB signaling pathway has been a target for pharmacological intervention, especially in models of inflammation or cancer, where the pathway is often constitutively active (1). Over 750 inhibitors of the NF-κB pathway have been identified, including both natural and synthetic molecules (1). Conversely, many different stimuli have been identified which activate the NF-κB pathway, including cytokines such as TNF-α and interleukin-1β, pathogenic bacteria and viruses, bacterial lipopolysaccharide and peptidoglycan, and oxidative stress. The NF-κB/293/GFP™ cell line allows the researcher to study both potential inhibitors and activators of the NF-κB pathway, and as such, is an extremely valuable tool for a wide variety of research applications.