Cholesterol Homeostasis Pathway Reporters

Liver X Receptors (LXRs) play a key role in lipid homeostasis. LXRs form heterodimers with RXR to bind to LXR response elements (LXREs) in the target promoter regions of cholesterol regulating genes. The LXRE is composed of two direct repeat hexanucleotide sequences, separated by four bases. LXREs are found in the promoters of key genes involved in cholesterol synthesis, transport, and metabolism, as well as transport and synthesis of fatty acids. LXRs are implicated as important sensors of sterol metabolism including the maintenance of normal cholesterol balance by promoting sterol efflux from peripheral cells. Increases in circulating HDL-cholesterol ultimately leads to hepatic sterol catabolism and excretion.

The LXR Response Cholesterol Homeostasis Pathway Reporters (shown in green boxes above) are designed to facilitate research into studying cholesterol efflux mechanisms and for developing target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis. Each construct is available as a lentivector plasmid, as prepackaged virus and as a stable reporter HepG2 cell line.

Example of GFP Data

Sample Luciferase Data