microRNA Cloning Vectors
Efficient and stable miRNA overexpression
Lentiviral-based microRNA expression
- Stably express any miRNA
- Create stable cell lines
- Choice of HIV & FIV vectors with fluorescent protein markers
HIV-based lentivectors
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Vector Maps |
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High levels of microRNA precursor expression from CMV promoter. The multiple cloning site (MCS) located downstream of a CMV promoter allows for convenient cloning of your microRNA presursor of interest. The SV40 polyadenylation signal in both vectors enables efficient termination of transcription, resulting in high levels of steady-state expression. Downstream of your expression cassette is an EF1 promoter driving the expression of either the Puro, copGFP or RFP marker genes. |
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FIV-based lentivectors |
| Description | Vector Maps |
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Express native microRNAs with the CMV promoter in target cells through transfection (plasmid form) or transduction (packaged constructs). |
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