Measure exact infectivity of virus with no inactive virus background
The UltraRapid Lentiviral Titer Kit is designed to rapidly determine the titers of pseudoviral particles that are generated with SBI’s FIV and HIV lentiviral vectors or libraries. It allows users to measure the copy numbers of integrated lentiviral constructs in genomic DNA of transduced target cells. Except for nuclease-free water, the kit contains all components necessary for measuring the amounts of endogenous PGK1 DNA and the pseudo-lentiviral-specific WPRE element that is integrated into the genomes of successfully tranduced cells in each sample. The titer of each sample is then determined by calculating the amount of WPRE element relative to that of PGK1 DNA against a standard curve generated with the provided calibration standards.
Sample Data
Sample A: amplification plot of PGK1 genomic DNA of calibration standards
Sample B: dissociation curves of the amplified PGK1
Sample C: amplification plot of WPRE DNA of the calibration standards
Sample D: dissociation curves of the amplified WPRE
Sample E: amplification plot of a sample experiment
