PEG-it Virus Concentration
TransDux Infection

Easily Concentrate Lentiviruses with PEG-it™ Virus Precipitation Solution

Easy to use and cost-effective method for highly efficient viral concentration

Concentrate all lentiviral particles 10- to 100-fold

Non-toxic reagents safe for use with all target cell lines tested, including ES cells

No special equipment required—avoid the expense of an ultracentrifuge

No accumulation of cellular debris like with ultracentrifugation—avoid toxic and immunogenic reactions by using PEG-it™ instead

Ideally suited for concentrating virus from large volumes —avoid the limited capacity per spin of ultracentrifugation

Improve transduction of cells and prepare ultra-high titer preps for in vivo infections

Sterile and ready for use in cell culture

Couple precipitated virus with TransDux™ for high infectivity

Completely compatible with System Bioscience’s LentiMag™ Magnetotransduction Kit

 



 

Concentrate viral particles 100-fold

PEG-it™ Virus Precipitation Solution provides a simple and highly effective means to concentrate lentiviral particles, such as those produced with System Biosciences' pPACK Lentivector Packaging System.  PEG-it™Virus Precipitation Solution is a formulation of polyethylene glycol optimized for the precipitation of all lentiviral-based particles.  The solution is mixed with virus-containing cell culture supernatant, incubated at 4°C overnight, and centrifuged at 1500 × g to pellet precipitated viral particles.  As a result, viral particle concentrations are increased 10- to 100-fold.

Non-toxic and Effective with Stem Cells

Sample Data of PEG-it concentrated lentivirus added to Human Stem Cells

Infect more cells with TransDux

Simply mix with virus to achieve superior transduction results

TransDux™ is a unique infection reagent that enables high transduction rates of virus into most cells, even those that are resistant to infections. TransDux is provided as a 200x solution, sufficient for 80 transductions for a 24 well plate (cat# LV850A-1).

Online Resources

Product Citations

Seungjin Shin and Lonnie D. Shea. Lentivirus Immobilization to Nanoparticles for Enhanced and Localized Delivery From Hydrogels. Molecular Therapy (2010) 18 4, 700–706. PDF>>>

Stefano Bartesaghi, Joanne Betts-Henderson, Kelvin Cain, David Dinsdale, Xiaoshan Zhou, Anna Karlsson, Paolo Salomoni1 and Pierluigi Nicotera. Loss of thymidine kinase 2 alters neuronal bioenergetics and leads to neurodegeneration. Hum Mol Genet. 2010 May 1;19(9):1669-77. PDF>>>

Seungjin Shin, Hannah M. Tuinstra, David M. Salvay and Lonnie D. Shea. Phosphatidylserine immobilization of lentivirus for localized gene transfer. Biomaterials Volume 31, Issue 15, May 2010, Pages 4353-4359.

Yonghui Zhang, Xiaojing Lin, Guoqin Wang, Jianfang Zhou, Jian Lu, Honglan Zhao, Fengwei Zhang, Jia Wu, Chunqiong Xu, Ning Du, Zi Li, Ye Zhang, Xiaoyi Wang, Shengli Bi, Yuelong Shu, Hongning Zhou, Wenjie Tan, Xiaobing Wu, Zhihui Chen, Yue Wang. Neuraminidase and Hemagglutinin Matching Patterns of a Highly Pathogenic Avian and Two Pandemic H1N1Influenza A Viruses. PLoS One. 2010; 5(2): e9167. PDF>>>

Ning Sun, Nicholas J. Panetta, Deepak M. Gupta, Kitchener D. Wilson, Andrew Lee, Fangjun Jia, Shijun Hu, Athena M. Cherry, Robert C. Robbins, Michael T. Longaker, and Joseph C. Wu. Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells. Proc Natl Acad Sci U S A. 2009 September 15; 106(37): 15720–15725. PDF>>>

Pintchovski SA, Peebles CL, Kim HJ, Verdin E, Finkbeiner S. The serum response factor and a putative novel transcription factor regulate expression of the immediate-early gene Arc/Arg3.1 in neurons. J Neurosci. 2009 Feb 4;29(5):1525-37.

Zhang Q, Huang A, Lin YC, Yu HG. Associated changes in HCN2 and HCN4 transcripts and I(f) pacemaker current in myocytes. Biochim Biophys Acta. 2009 Feb 21.

Badri KR, Zhou Y, Dhru U, Aramgam S, Schuger L. Effects of the SANT domain of tension-induced/inhibited proteins (TIPs), novel partners of the histone acetyltransferase p300, on p300 activity and TIP-6-induced adipogenesis. Mol Cell Biol. 2008 Oct;28(20):6358-72.

PEG-it™ Virus Concentration Solution Quantity Product Size Catalog # Price PEG-it Virus Precipitation Solution (100 mL aliquot) 100 mL LV810A-1 $253.50 PEG-it Virus Precipitation Solution (250 mL aliquot) 250 mL LV825A-1 $552.50 TransDux virus transduction reagent (200x) 80 transductions LV850A-1 $190.00 * 100 ml of sterile PEG-it™ reagent is sufficient for precipitation of lentiviral particles from 400 ml of cell culture supernatant. ** 250 ml of sterile PEG-it™ reagent is sufficient for precipitation of lentiviral particles from 1L of cell culture supernatant. *** TransDux is provided at a 200x concentration, 200ul (80 transductions for 24 well plates).

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