LentiMag™

Magneto Lentiviral Transduction

The LentiMag™ kit provides a novel, simple, and highly effective approach to increase the number of cells positively transduced with System Bioscience’s HIV- and FIV-based lentiviral vectors compared to transduction aided by addition of polybrene. LentiMag™ is a nanoparticle formulation specifically developed for transduction of mammalian cells using Retrovirus- or Lentivirus-based gene and siRNA delivery vectors.

Increase Transduction Efficiency with LentiMag™

Increase percentage of cells transduced with System Bioscience’s lentiviral vector compared to transductions performed alone or with polybrene
Concentrate lentiviral vector dose onto cells very rapidly
Successfully transduce nonpermissive (difficult-to-infect) cells and cell lines
Compatible with adherent cells and cells grown in suspension
Significantly improve transduction with low titer lentiviral vector doses
Economical, simple to use, and rapid
Nontoxic; does not require the use of polybrene
Sterile; ready for use in cell culture
Can be used with both Lentiviruses & retroviruses from a number of sources

Lentiviral vectors are complexed with magnetic nanoparticles, which are then placed within a strong magnetic field to rapidly pull the virus particles towards the target cells. In this manner, the applied viral dose is concentrated onto cells within minutes so that most of the cells come into contact with the lentiviral vector inoculum. As a result, the number of transduced cells is increased by up to 3-fold as compared to traditional transductions performed using polybrene (Fig. 1 a&b).

LentiMag™ reagent requires a strong magnetic field to enhance transduction of target cells with lentiviral vectors. The magnetic plate supplied with the kit has been specifically designed to exert this magnetic field. The geometry of the plate is compatible with all standard cell culture dishes, including T-25 and T-75 flasks, 60 and 100 mm diam. dishes, and 6-, 12-, 24-, 48-, & 96-well plates.

Fig. 1a

 

Fig. 1b

 

Online Resources

 

Technical References

Wells DJ. Gene therapy progress and prospects: electroporation and other physical methods. Gene Ther. 2004 Sep;11(18):1363-9. PDF>>>

F Scherer, M Anton, U Schillinger, J Henke, C Bergemann, A Kru¨ger, B Ga¨nsbacher and C Plank. Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo. Gene Therapy (2002) 9, 102–109. PDF>>>

LentiMag Magneto Transduction Quantity Product Size Catalog # Price LentiMag Magneto Transduction Kit 10 transductions LV800A-1 $539.00 LentiMag Reagent 10 transductions LV801A-1 $330.00 SBI LentiMag™ kit: Includes 50 μl sterile LentiMag™ reagent (10 transductions in a 24-well plate format) and one magnetic plate. LentiMag™ Reagent only: For customers who have already purchased magnetic plate in above kit. Sterile, 50 μl  (10 transductions in a 24-well plate format).

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