Choose from a variety of cumate switch vector formats

SparQ All-in-one Cumate Switch Vectors
The All-in-one cumate switch inducible lentivector enables a simple way to make stable cell lines (using the Puro) marker and to turn on the switch by adding cumate. The inserted gene or microRNA at the MCS will be expressed upon induction (QM800A-1) or co-induced with IRES-mediated GFP marker to monitor induction when the switch is turned ON (QM812A-1). NOTE: Due to the size of the QM812A-1 lentivector, you are limited to inserting a cDNA into the MCS only up to 2kb in size. The Western blot and GFP data below are from a 1.3kb mouse Foxp3 cDNA in QM812A-1 with 300ug/ml cumate induction.

SparQ IRES Vectors
The IRES vector configuration enables the inducible expression of a microRNA or a cDNA along with an IRES-mediated GFP or RFP marker that wil be co-expressed to monitor induction when the switch is turned ON.

SparQ T2A Vectors
The T2A vector configuration enables the inducible expression of a cDNA along with the simultaneous, T2A-mediated GFP or RFP marker to monitor cumate switch induction.

SparQ Dual Promoter Vectors
The dual promoter vector configuration enables the inducible expression of a microRNA or a cDNA. The downstream EF1 promoter will constitutively express GFP (QM511B-1) or RFP (QM512B-1), or the GFP+Puro (QM513B-1), RFP+Puro (QM516B-1) markers independent of the cumate switch activation.

Choose a CymR Repressor Lentivector Format

CymR Repressor Vectors
The system requires that the CymR repressor is present. Co-transduce the CymR vector before induction or establish an inducer cell line prior to induction studies. Use either the Puromycin or RFP CymR format to monitor CymR positive cells.