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How to Make High Titer Lentivirus

Efficient delivery:  Engineered lentiviral vectors enable the efficient and robust delivery of genetic material to most cell types, including non-dividing and hard-to-transfect cells (primary, blood, stem cells) in vitro or to transgenic mice models in vivo

Stable, heritable expression:  Constructs integrated into genomic DNA - High expression levels in chromosomal environment, with low variability

Non-disruptive to cells:  Cell functions are unaffected by the infection process

Biosafe:  Replication-incompetent design prevents proliferation of virus for both HIV and non-human FIV lentivectors

System Biosciences (SBI) offers an extended set of cloning and expression lentivectors for efficient delivery of expression constructs in mammalian systems. To achieve highly efficient delivery and stable expression, the lentiviral constructs can be packaged in VSV-G pseudoviral particles and transduced into a wide range of cell lines or to model organisms (mouse, rat, etc.).

In comparison to traditional retroviral delivery systems, lentivectors actively integrate into genomic DNA without requiring cell replication. SBI offers lentivectors derived from commonly-used HIV (human immunodeficiency virus) and FIV (feline immunodeficiency virus). Both types of lentivectors have similar structures and performance, but may have different transduction efficiencies for different cell types (See sample transduction data, pdf). Although higher titers are usually achieved with HIV-based vectors, FIV-based vectors are considered a biologically-safe alternative as they are derived from a non-human feline virus.

One of SBI’s main goals is to provide our customers with highly effective lentiviral vectors and ready-to-use, pre-packaged lentiviral constructs in VSV-G pseudoviral particles to make lentivector technology user-friendly & biosafe.