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Six iPSC Factors for Stem Cell pluripotency reprogramming –
Human clones ready-made and expression validated.
Features and Benefits
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Lentiviral-based protein expression system, HIV backbone for
high viral titers
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Pluripotency factors expressed from constitutive EF1α
promoter
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Reliable delivery to dividing or non-dividing cells
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Monitor positive expressing cells with co-expressed RFP
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All proteins are N-terminal HA-tagged
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Convenient sorting of transfected or transduced cells
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Create stable cell lines
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Monitor positive cells with RFP
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All 6 iPSC factors expression-validated
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Transfection Data
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Protein expression Western Blot Data
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The piPSC constructs can be used in transient transfection
experiments (shown above) or can be used to package lentiviral
particles. The particles can then be used to transduce any cell
type to create stable cell lines permanently expressing the iPSC
protein of interest. Examples of VSV-g pseudotyped lentiviral
transductions are shown below.
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Transduce Dividing and Non-dividing cells
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piPSC-Nanog activates Nanog reporter transcription
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Lentiviral transduction data
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Nanog reporter cell line transactivation data
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Kit Components
Each kit contains 10 μg
of endotoxin-free plasmid DNA in TE buffer or 1 x 106
ifu/ml pre-packaged lentivirus.
Each kit comes with a product certificate of analysis.
Ordering
Information
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Product |
Catalog # |
Virus Price |
Plasmid
Price |
To Order |
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piPSC-hNanog |
SR10060VA-1/PA-1 |
$495 |
$1,295 |
order |
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piPSC-hOct4 |
SR10061VA-1/PA-1 |
$495 |
$1,295 |
order |
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piPSC-hSox2 |
SR10062VA-1/PA-1 |
$495 |
$1,295 |
order |
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piPSC-hcMyc |
SR10063VA-1/PA-1 |
$495 |
$1,295 |
order |
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piPSC-hLin28 |
SR10064VA-1/PA-1 |
$495 |
$1,295 |
order |
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piPSC-hKLF4 |
SR10065VA-1/PA-1 |
$495 |
$1,295 |
order |
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piPSC-entire set |
SR10066VA-1/PA-1 |
$2,900 |
$7,700 |
order |
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VA-1
product version refers to virus (1 x 106 ifu/ml)
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PA-1
product version refers to plasmid (10
μg)
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piPSC-entire
set comes with 6 individual plasmids or 6 individual
pre-packaged lentiviral preparations.
To obtain more information contact SBI at 888-266-5066
(toll-free in North America) or 650-968-2200, or e-mail to
orders@systembio.com
Details of the Lentiviral Delivery System:
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Express iPSC protein factors in target cells through
transfection (plasmid form) or transduction (packaged
constructs).
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Simple packaging into pseudoviral particles with SBI’s pPACK
Lentivector Packaging System (see:
http://www.systembio.com/pPACK.htm)
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Deliver piPSC expression constructs into wide range of cell
lines including non-dividing or difficult-to-transfect cell
lines with biologically-safe and high-titer enabled HIV-based
lentivector.
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RNA polymerase II driven expression of iPSC protein factors
offers high level of expression from single copy of insert.
Related Products
Stem Cell Differentiation Reporters
PathNet™ Transcriptional Reporter Lentivectors
Transcriptional Reporter Cell Lines
GeneNet™ siRNA Libraries
References
Pluripotency Factors
Rossant, J. 2007. Stem cells: The magic brew. Nature 448,
260-262.
Takahashi, K. and Yamanaka, S. 2006. Induction of pluripotent
stem cells from mouse embryonic and adult fibroblast cultures by
defined factors. Cell 126: 663–676.
Takahashi K. et al. 2007. Induction of pluripotent stem cells
from adult human fibroblasts by defined factors. Cell.
131: 861-72.
Park, IH et al., 2008. Reprogramming of human somatic cells to
pluripotency with defined factors. Nature.
451:141-6.
Baker, Monya 2007. Adult cells reprogrammed to pluripotency,
without tumors. Nature Reports Stem Cells. 2007-12-11.
Nakagawa, M et al., 2008. Generation of induced pluripotent stem
cells without Myc from mouse and human fibroblasts. Nature
Biotechnology. 26: 101-106.
Okita, K, et. al. 2007. Generation of germline-competent induced
pluripotent stem cells. Nature. 448:313-7
Yu, J. et al. 2007. Induced Pluripotent Stem Cell Lines Derived
from Human Somatic Cells.
Science
318: 1917 – 1920. |
