GeneNet™ Lentiviral siRNA Libraries

  • Screen genome-wide or pathway-focused for genes regulating cellular responses or signaling pathways

  • Identify potential drug targets or diagnostic markers that inhibit, enhance, or activate a selectable phenotype

  • Find crucial regulatory genes using routine growth selections or flow sorting

  • High-throughput identification of functional siRNAs by Affymetrix GeneChip® hybridization

  • Cost-effective, high-throughput RNAi functional analysis at prices affordable
    to the average researcher

  • Choose from siRNA libraries in FIV or HIV-based lentivectors

  • Also available in plasmid form, you can now produce large quantities of packaged siRNA library in your own lab

GeneNet™ siRNA Libraries enable you to perform high-throughput gene knockdown studies on a genome-wide or pathway-focused basis. You can simultaneously identify multiple genes that alter a specific cellular phenotype—in a single experiment.

By merging effector expression libraries with siRNA technology and the efficiency of lentiviral transduction, GeneNet™ siRNA Libraries enable you to conveniently introduce and see the effect of many thousands of siRNA molecules on cell functions at once. As outlined on the page describing how the library works, after selecting transduced cells that express a desired phenotype (such as resistance to apoptosis or irradiation, or a growth response to a chemical additive, etc.), you can identify the sets of genes that control the response.

Developed to be an affordable approach for the average researcher, GeneNet™ siRNA Libraries cost much less than you might think.

GeneNet™ Genome-wide
siRNA Library

 Vector # Target
Genes		 # siRNAs Cat. #
(Packaged)		 Cat. #
(Plasmid)
Human 8.5K pSIF1-H1-Puro 8,500 43,800 SI202B-1 SI202PB-1
Human 50K pSIF1-H1-copGFP 47,400 200,000 SI205A-1 SI205PB-1
Human 50K pSIF1-H1-Puro 47,400 200,000 SI206B-1 SI206PB-1
Human 50K pSIH1-H1-Puro 47,400 200,000 SI606B-1 SI606PB-1
Mouse 40K pSIF1-H1-Puro 39,000 150,000 SI222B-1 SI222PB-1
Mouse 40K pSIH1-H1-Puro 39,000 150,000 SI622B-1 SI622PB-1
           

GeneNet™ Focused
siRNA Library

 Vector # Target
Genes		 # siRNAs Cat. #
(Packaged)		 Cat. #
(Plasmid)
Human Apoptosis pSIH1-H1-Puro 579 6,876 SI616VA-1 SI616PA-1
Human Kinase pSIH1-H1-Puro 897 10,453 SI626VA-1 SI626PA-1
Human Phosphatase pSIH1-H1-Puro 244 2,719 SI636VA-1 SI636PA-1

(click on library name to get more information)

Sample Library Data

A.    Screening for Genes Involved in Fas-induced apoptosis

(Click image for PDF version)

 


This figure demonstrates the use of SBI's genome-wide siRNA library for the search for genes involved in Fas-induced apoptosis.  Red circles represent siRNAs that are enriched only in the Fas-treated samples, and therefore may silence gene(s) associated with the biological pathway(s) in vivo that are associated with apoptosis.  Blue circles are siRNAs present in both Fas-treated and control samples.
 

B. Screening for Genes Associated with Differential Response to a Drug

(Click image for PDF version)


This figure demonstrates the use of SBI’s genome-wide library to identify genes associated with drug response.

The “Eliminated siRNAs” correspond to those genes that conferred a selective disadvantage in presence of the drug and hence are only present in the Control population.

The “Enriched siRNAs” correspond to those genes that confer a selective advantage in presence of the drug (perhaps enabling cell survival) and hence are only present in the Drug Treated population.
 


Relevant publications:
 Fred Hutchinson Cancer Research Center (FHCRC) Manuscript PDF File

Hattori H, Zhang X, Jia Y, Subramanian KK, Jo H, Loison F, Newburger PE,
Luo HR. RNAi screen identifies UBE2D3 as a mediator of all-trans retinoic acid-induced cell growth arrest in human acute promyelocytic NB4 cells.
Blood, 110 (2): 640-650, 2007.

 

How to use siRNA libraries for genetic screening:
 Nature. 2004 Mar 25;428(6981):427-31
Nature. 2004 Mar 25;428(6981):431-7
Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):135-40
Nature 2006

SiRNA Library Review:
Click to download PDF